Detekce kojeneckých fekálních bifidobakterií pomocí enzymatické metody.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A12482" target="_blank" >RIV/60460709:41210/05:12482 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/05:00027355 RIV/00064203:_____/05:00000130 RIV/00216208:11130/05:00004187

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of infant faecal bifidobacteria by enzymatic methods.

Popis výsledku v původním jazyce

An enzyme-based assay was developed for the detection of bifidobacteria in infant faeces. Ninety-five samples from 51 breastfed infants in the age between 3 and 276 days were investigated. Bifidobacteria and other bacterial groups were determined by cultivation and fluorescence in situ hybridisation (FISH). Faecal samples were examined for the activity of fructoso-6-phosphate phosphoketolase and for other enzymatic reactions using the API-ZYM kit.Twenty-nine infants had high numbers of bifidobacteria (usually higher than 9 log CFU/g) in their faeces. Seventeen infants (35%) did not contain detectable amounts of bifidobacteria in their faecal samples. The remaining five individuals had low counts of bifidobacteria (3 to 6 log CFU/g). Most negative infants possessed major amounts of clostridia in their faecal flora. There were no significant differences among bifidobacterial counts obtained by cultivation and FISH, detection of F6PPK, &#945;-galactosidase and &#945;-glucosidase activitie

Název v anglickém jazyce

Detection of infant faecal bifidobacteria by enzymatic methods.

Popis výsledku anglicky

An enzyme-based assay was developed for the detection of bifidobacteria in infant faeces. Ninety-five samples from 51 breastfed infants in the age between 3 and 276 days were investigated. Bifidobacteria and other bacterial groups were determined by cultivation and fluorescence in situ hybridisation (FISH). Faecal samples were examined for the activity of fructoso-6-phosphate phosphoketolase and for other enzymatic reactions using the API-ZYM kit.Twenty-nine infants had high numbers of bifidobacteria (usually higher than 9 log CFU/g) in their faeces. Seventeen infants (35%) did not contain detectable amounts of bifidobacteria in their faecal samples. The remaining five individuals had low counts of bifidobacteria (3 to 6 log CFU/g). Most negative infants possessed major amounts of clostridia in their faecal flora. There were no significant differences among bifidobacterial counts obtained by cultivation and FISH, detection of F6PPK, &#945;-galactosidase and &#945;-glucosidase activitie

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiolocal Methods

ISSN

0167-7012

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

99

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

309-312

Kód UT WoS článku

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EID výsledku v databázi Scopus

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