První nález viroidu latentní mozaiky broskvoně v Egyptě

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F08%3A26261" target="_blank" >RIV/60460709:41210/08:26261 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

First report of Peach latent mosaic viroid infecting peach in Egypt

Popis výsledku v původním jazyce

Peach latent mosaic viroid (PLMVd) is a widespread pathogen of stone fruit trees in some European and Mediterranean countries, and also in Northern America. To access the presence of the viroid in Egypt, a survey was conducted covering five commercial peach orchards in El Khatatba region in Al Minufiya governorate. During 2003-2004, 73 peach trees (cv. Florida grafted on Nemagard rootstock) were visually inspected and sampled. No symptoms characteristic of PLMVd infection as mosaic, delayed vegetation or fruit suture cracking were observed. All samples were tested for the presence of PLMVd using dot-blot hybridization and reverse transcription-polymerase chain reaction (RT-PCR). Aliquots (5 ?l) of total nucleic acids, extracted from about 2 mg of leaftissues, were spotted onto positively charged nylon membranes and hybridized under stringent conditions using a digoxigenin-labeled riboprobe (1). The extracts (1 ?l) also were used in RT-PCR as described by Loreti et al. (2). Only one of

Název v anglickém jazyce

First report of Peach latent mosaic viroid infecting peach in Egypt

Popis výsledku anglicky

Peach latent mosaic viroid (PLMVd) is a widespread pathogen of stone fruit trees in some European and Mediterranean countries, and also in Northern America. To access the presence of the viroid in Egypt, a survey was conducted covering five commercial peach orchards in El Khatatba region in Al Minufiya governorate. During 2003-2004, 73 peach trees (cv. Florida grafted on Nemagard rootstock) were visually inspected and sampled. No symptoms characteristic of PLMVd infection as mosaic, delayed vegetation or fruit suture cracking were observed. All samples were tested for the presence of PLMVd using dot-blot hybridization and reverse transcription-polymerase chain reaction (RT-PCR). Aliquots (5 ?l) of total nucleic acids, extracted from about 2 mg of leaftissues, were spotted onto positively charged nylon membranes and hybridized under stringent conditions using a digoxigenin-labeled riboprobe (1). The extracts (1 ?l) also were used in RT-PCR as described by Loreti et al. (2). Only one of

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QG60123" target="_blank" >QG60123: Výzkum a inovace postupů diagnostiky hospodářsky významných, regulovaných a karanténních fytopatogenních organismů pro systém certifikace ovocných dřevin s důrazem na molekulární metody</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Disease

ISSN

0191-2917

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

92

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

1

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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