Biological characterization and variability in the coat protein gene of an isolate of Apricot latent virus.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F10%3A49040" target="_blank" >RIV/60460709:41210/10:49040 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Biological characterization and variability in the coat protein gene of an isolate of Apricot latent virus.

Popis výsledku v původním jazyce

Virus-free accessions of peach, apricot, cherry and plum were graft-inoculated in 2004 with buds from a peach tree cv. Missour infected by Apricot latent virus (ApLV), and then analysed by RT-PCR using a specific primer set. The expected DNA fragment of200 bp in size was amplified from 33 different Prunus sources, thus extending the woody host range of ApLV to new cultivars of Prunus persica, P. avium, P. armeniaca and for the first time to European (Prunus domestica) and Japanese (P. salicina) plum cultivars. Several peach cultivars and apricot cv. Tirynthos were symptomatic. To determine the best source material for ApLV detection, RT-PCR assays were performed during the growing season. ApLV was homogeneously distributed in flowers, leaves, petioles, barks and fruits of peach and apricot but was detected only in leaves of cherry and plum. The genetic variability of the ApLV coat protein gene from graft-inoculated hosts and corresponding sequences from databases was 63.5-100%.

Název v anglickém jazyce

Biological characterization and variability in the coat protein gene of an isolate of Apricot latent virus.

Popis výsledku anglicky

Virus-free accessions of peach, apricot, cherry and plum were graft-inoculated in 2004 with buds from a peach tree cv. Missour infected by Apricot latent virus (ApLV), and then analysed by RT-PCR using a specific primer set. The expected DNA fragment of200 bp in size was amplified from 33 different Prunus sources, thus extending the woody host range of ApLV to new cultivars of Prunus persica, P. avium, P. armeniaca and for the first time to European (Prunus domestica) and Japanese (P. salicina) plum cultivars. Several peach cultivars and apricot cv. Tirynthos were symptomatic. To determine the best source material for ApLV detection, RT-PCR assays were performed during the growing season. ApLV was homogeneously distributed in flowers, leaves, petioles, barks and fruits of peach and apricot but was detected only in leaves of cherry and plum. The genetic variability of the ApLV coat protein gene from graft-inoculated hosts and corresponding sequences from databases was 63.5-100%.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QG60123" target="_blank" >QG60123: Výzkum a inovace postupů diagnostiky hospodářsky významných, regulovaných a karanténních fytopatogenních organismů pro systém certifikace ovocných dřevin s důrazem na molekulární metody</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Plant Pathology

ISSN

1125-4653

e-ISSN

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Svazek periodika

92

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000276175100011

EID výsledku v databázi Scopus

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