The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F13%3A59827" target="_blank" >RIV/60460709:41210/13:59827 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027014:_____/13:#0001782

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

Popis výsledku v původním jazyce

Nitric oxide (NO) and protein kinase C (PKC) are involved in the activation of mammalian oocytes, although their role in the exit from the metaphase II stage and cortical granules (CG) exocytosis is still not fully understood. The aim of this study was to verify whether NO-donor together with specific PKC-activators induce the complete activation of porcine oocytes assessed as meiosis resumption and a cortical reaction. Pig maturated oocytes were treated with the NO-donor S-nitroso-N-acetylpenicillamine(SNAP) or PKC-activators such as phorbol-12-myristate-13-acetate (PMA), 1-oleyl-2-acetyl-sn-glycerol (OAG) and L-?-phosphatidylinositol-3,4,5-trisphosphate dipalmitoyl heptaammonium salt (DPAM). To study the combined effect of NO-donor and PKC-activators, aliquots of oocytes were also incubated with SNAP together with PKC-activators (SNAP-DPAM, SNAP-OAG and SNAP-PMA groups). The results showed that 0% of control oocytes reassumed meiosis. However, both the PKC-activators (DPAM 44.0 ? 10

Název v anglickém jazyce

The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

Popis výsledku anglicky

Nitric oxide (NO) and protein kinase C (PKC) are involved in the activation of mammalian oocytes, although their role in the exit from the metaphase II stage and cortical granules (CG) exocytosis is still not fully understood. The aim of this study was to verify whether NO-donor together with specific PKC-activators induce the complete activation of porcine oocytes assessed as meiosis resumption and a cortical reaction. Pig maturated oocytes were treated with the NO-donor S-nitroso-N-acetylpenicillamine(SNAP) or PKC-activators such as phorbol-12-myristate-13-acetate (PMA), 1-oleyl-2-acetyl-sn-glycerol (OAG) and L-?-phosphatidylinositol-3,4,5-trisphosphate dipalmitoyl heptaammonium salt (DPAM). To study the combined effect of NO-donor and PKC-activators, aliquots of oocytes were also incubated with SNAP together with PKC-activators (SNAP-DPAM, SNAP-OAG and SNAP-PMA groups). The results showed that 0% of control oocytes reassumed meiosis. However, both the PKC-activators (DPAM 44.0 ? 10

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/GAP502%2F10%2F0794" target="_blank" >GAP502/10/0794: Zisk meiotické kompetece prasečích oocytů ? role protein fosfatázy kalcineurinu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal

ISSN

1751-7311

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

279-286

Kód UT WoS článku

000313218900012

EID výsledku v databázi Scopus

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