Comparative molecular analysis of Bursaphelenchus vallesianus, a wood-inhabiting nematode isolated from declining pine trees in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F14%3A60882" target="_blank" >RIV/60460709:41210/14:60882 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparative molecular analysis of Bursaphelenchus vallesianus, a wood-inhabiting nematode isolated from declining pine trees in the Czech Republic

Popis výsledku v původním jazyce

The Bursaphelenchus genus (Nematoda: Parasitaphelenchidae) comprises mostly wood-inhabiting nematodes that feed on various tree-colonizing fungi. One species of the genus, B. xylophilus, has been proven as an agent causing pine wilt disease (PWD). However, involvement of other Bursaphelenchus species in the PWD remains enigmatic. In the current paper, comparative molecular analysis is performed based on nuclear ribosomal DNA (rDNA) of B. vallesianus, a species that was recently isolated from pine trees(Pinus sylvestris) exhibiting wilting and declining symptoms in the Czech Republic. Sequencing of the nuclear-encoded ITS1?5*8S?ITS2 rDNA region confirmed previous taxonomic conclusions based on morphology. Evolutionary reconstructions resulted in a phylogenetic tree, where the Czech isolate of B. vallesianus occupied a common clade together with other species belonging to the so-called B. sexdentati group. Unexpectedly, comprehensive analysis of the sequence data revealed a genetic vari

Název v anglickém jazyce

Comparative molecular analysis of Bursaphelenchus vallesianus, a wood-inhabiting nematode isolated from declining pine trees in the Czech Republic

Popis výsledku anglicky

The Bursaphelenchus genus (Nematoda: Parasitaphelenchidae) comprises mostly wood-inhabiting nematodes that feed on various tree-colonizing fungi. One species of the genus, B. xylophilus, has been proven as an agent causing pine wilt disease (PWD). However, involvement of other Bursaphelenchus species in the PWD remains enigmatic. In the current paper, comparative molecular analysis is performed based on nuclear ribosomal DNA (rDNA) of B. vallesianus, a species that was recently isolated from pine trees(Pinus sylvestris) exhibiting wilting and declining symptoms in the Czech Republic. Sequencing of the nuclear-encoded ITS1?5*8S?ITS2 rDNA region confirmed previous taxonomic conclusions based on morphology. Evolutionary reconstructions resulted in a phylogenetic tree, where the Czech isolate of B. vallesianus occupied a common clade together with other species belonging to the so-called B. sexdentati group. Unexpectedly, comprehensive analysis of the sequence data revealed a genetic vari

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QG60123" target="_blank" >QG60123: Výzkum a inovace postupů diagnostiky hospodářsky významných, regulovaných a karanténních fytopatogenních organismů pro systém certifikace ovocných dřevin s důrazem na molekulární metody</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Pathology

ISSN

0032-0862

e-ISSN

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Svazek periodika

63

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

232-242

Kód UT WoS článku

000329329500025

EID výsledku v databázi Scopus

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