Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F14%3A64334" target="_blank" >RIV/60460709:41210/14:64334 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner

Popis výsledku v původním jazyce

The aim of the study was to determine the influence of different concentrations of phytoestrogens biochanin A (BIO A; 20, 40, 50?g ml?1) and daidzein (DAI; 10, 20, 40, 50?g ml?1) on the course of meiotic maturation of pig oocytes. After a 24-hour cultivation, a stage of nuclear maturation was achieved and the areas of cumulus-oocyte complexes (COCs), as an indicator of cumulus expansion, were evaluated. The effects of both contaminants on oocytes were mani - fested from the lowest concentration used. Nuclear maturation was inhibited in a dose-dependent manner in the case of BIO A. Effects of DAI reached a plateau at a concentration of 20?g ml?1.Possible relationship to limited solubility of DAI was excluded because limits of DAI solubility in culture medium were confirmed at 50 ?g ml?1.The cumulus expansion was also influenced in a different manner ? reduction of the COC?s area by BIO A was dose-dependent, whereas DAI had the strongest effect on CCs in the lowest and highest concentrat

Název v anglickém jazyce

Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner

Popis výsledku anglicky

The aim of the study was to determine the influence of different concentrations of phytoestrogens biochanin A (BIO A; 20, 40, 50?g ml?1) and daidzein (DAI; 10, 20, 40, 50?g ml?1) on the course of meiotic maturation of pig oocytes. After a 24-hour cultivation, a stage of nuclear maturation was achieved and the areas of cumulus-oocyte complexes (COCs), as an indicator of cumulus expansion, were evaluated. The effects of both contaminants on oocytes were mani - fested from the lowest concentration used. Nuclear maturation was inhibited in a dose-dependent manner in the case of BIO A. Effects of DAI reached a plateau at a concentration of 20?g ml?1.Possible relationship to limited solubility of DAI was excluded because limits of DAI solubility in culture medium were confirmed at 50 ?g ml?1.The cumulus expansion was also influenced in a different manner ? reduction of the COC?s area by BIO A was dose-dependent, whereas DAI had the strongest effect on CCs in the lowest and highest concentrat

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia Agriculturae Bohemica

ISSN

1211-3174

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

155-161

Kód UT WoS článku

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EID výsledku v databázi Scopus

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