The yield and cell viability of bovine in vivo recovered embryos in relation to season of flushing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F14%3A65576" target="_blank" >RIV/60460709:41210/14:65576 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The yield and cell viability of bovine in vivo recovered embryos in relation to season of flushing

Popis výsledku v původním jazyce

The objective of this study was to evaluate effect of the season on the yield and viability of embryos recovered from superovulated and flushed Holstein cows (n = 56). The embryo recovery rate (ERR, %) was determined as the proportion of totally flushedembryos (TFE, n) to the number of corpora lutea detected. The embryos were evaluated as transferable (TRANS) or non-transferable and analysed for occurrence of apoptotic cells (T+, TUNELindex) as well as actin cytoskeleton quality (ACG). TFE and ERR werethe highest during spring vs summer (149 embryos, 59.6% vs 20 embryos, 37%; P < 0.001), while they insignificantly differed in autumn (57 embryos, 48.3%). The autumn embryos presented a higher proportion of TRANS (P < 0.05, +10.56% vs spring; +18.94% vssummer). The occurrence of apoptosis in these embryos was lower (?1.38 T+ cells and ?1.75% T-index; P < 0.05) and the percentage of the best ACG was higher (+35%; P < 0.05). These findings identified autumn as the best season for collect

Název v anglickém jazyce

The yield and cell viability of bovine in vivo recovered embryos in relation to season of flushing

Popis výsledku anglicky

The objective of this study was to evaluate effect of the season on the yield and viability of embryos recovered from superovulated and flushed Holstein cows (n = 56). The embryo recovery rate (ERR, %) was determined as the proportion of totally flushedembryos (TFE, n) to the number of corpora lutea detected. The embryos were evaluated as transferable (TRANS) or non-transferable and analysed for occurrence of apoptotic cells (T+, TUNELindex) as well as actin cytoskeleton quality (ACG). TFE and ERR werethe highest during spring vs summer (149 embryos, 59.6% vs 20 embryos, 37%; P < 0.001), while they insignificantly differed in autumn (57 embryos, 48.3%). The autumn embryos presented a higher proportion of TRANS (P < 0.05, +10.56% vs spring; +18.94% vssummer). The occurrence of apoptosis in these embryos was lower (?1.38 T+ cells and ?1.75% T-index; P < 0.05) and the percentage of the best ACG was higher (+35%; P < 0.05). These findings identified autumn as the best season for collect

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/QI91A061" target="_blank" >QI91A061: Vliv rozdílné metabolické aktivity organismu na biologické a oplodňovací charakteristiky gamet dojeného skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Animal and Feed Sciences

ISSN

1230-1388

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

8

Strana od-do

309-316

Kód UT WoS článku

000347504000004

EID výsledku v databázi Scopus

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