Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F14%3A66554" target="_blank" >RIV/60460709:41210/14:66554 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41340/14:66554

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization

Popis výsledku v původním jazyce

The major risk factor for Clostridium difficile infection is the use of antibiotics owing to the disruption of the equilibrium of the host gut microbiota. To preserve the beneficial resident probiotic bacteria during infection treatment, the use of molecules with selective antibacterial activity enhances the efficacy by selectively removing C. difficile. One of them is the plant alkaloid 8-hydroxyquinoline (8HQ), which has been shown to selectively inhibit clostridia without repressing bifidobacteria. Selective antimicrobial activity is generally tested by culture techniques of individual bacterial strains. However, their main limitation is the inability to describe differential growth dynamics of more bacterial strains cultured together within the same experiment. In the present study we have combined fluorescent in situ hybridization and flow cytometry to describe the changes in active and non-active cells of a mixed culture formed by the opportunistic pathogen C. difficile CECT 531

Název v anglickém jazyce

Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization

Popis výsledku anglicky

The major risk factor for Clostridium difficile infection is the use of antibiotics owing to the disruption of the equilibrium of the host gut microbiota. To preserve the beneficial resident probiotic bacteria during infection treatment, the use of molecules with selective antibacterial activity enhances the efficacy by selectively removing C. difficile. One of them is the plant alkaloid 8-hydroxyquinoline (8HQ), which has been shown to selectively inhibit clostridia without repressing bifidobacteria. Selective antimicrobial activity is generally tested by culture techniques of individual bacterial strains. However, their main limitation is the inability to describe differential growth dynamics of more bacterial strains cultured together within the same experiment. In the present study we have combined fluorescent in situ hybridization and flow cytometry to describe the changes in active and non-active cells of a mixed culture formed by the opportunistic pathogen C. difficile CECT 531

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF MEDICAL MICROBIOLOGY

ISSN

0022-2615

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

63

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

1663-1669

Kód UT WoS článku

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EID výsledku v databázi Scopus

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