Effect of LDL addition into selected bull sperm diluters on resistance of spermatozoa against cold shock

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F16%3A68160" target="_blank" >RIV/60460709:41210/16:68160 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.11118/actaun201664020395" target="_blank" >http://dx.doi.org/10.11118/actaun201664020395</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11118/actaun201664020395" target="_blank" >10.11118/actaun201664020395</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of LDL addition into selected bull sperm diluters on resistance of spermatozoa against cold shock

Popis výsledku v původním jazyce

The aim of work was to determine the effect of LDL cholesterol addition into selected diluents on the resistance of spermatozoa to cold shock and on their short-term survivability during cold test. The hypothesis was that the addition of LDL cholesterol will positively affects sperm resistance to cold shock and ensures a higher survivability of spermatozoa during short-term cold survival test. Four bulls of different breeds and ages, from the same A.I. centre were used. A total of eight semen collections were processed. Each ejaculate was divided into 6 portions (3 controls and 3 samples). Three commercially produced extenders, AndroMed, Bioxcell, and Triladyl 40 were used, each in standard and LDL enriched variants. In the case of AndroMed or Bioxcell, 6% of LDL was simply added. In Triladyl, 10% of LDL replaced the standard egg yolk component. Spermatozoa resistance to cold shock was evaluated by the percentage of live sperm using Eosin-Nigrosine staining. The results showed the influence of bull

Název v anglickém jazyce

Effect of LDL addition into selected bull sperm diluters on resistance of spermatozoa against cold shock

Popis výsledku anglicky

The aim of work was to determine the effect of LDL cholesterol addition into selected diluents on the resistance of spermatozoa to cold shock and on their short-term survivability during cold test. The hypothesis was that the addition of LDL cholesterol will positively affects sperm resistance to cold shock and ensures a higher survivability of spermatozoa during short-term cold survival test. Four bulls of different breeds and ages, from the same A.I. centre were used. A total of eight semen collections were processed. Each ejaculate was divided into 6 portions (3 controls and 3 samples). Three commercially produced extenders, AndroMed, Bioxcell, and Triladyl 40 were used, each in standard and LDL enriched variants. In the case of AndroMed or Bioxcell, 6% of LDL was simply added. In Triladyl, 10% of LDL replaced the standard egg yolk component. Spermatozoa resistance to cold shock was evaluated by the percentage of live sperm using Eosin-Nigrosine staining. The results showed the influence of bull

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210109" target="_blank" >QJ1210109: Inovace zpracování ejakulátu býků a technologických postupů výroby inseminačních dávek vedoucí ke zvýšení jejich oplozovací schopnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

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Svazek periodika

64

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

395-399

Kód UT WoS článku

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EID výsledku v databázi Scopus

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