Purification of a Fe-SOD excreted byLeishmania braziliensisfor specific antibodies detection in Mexican human sera:Cutting-edge the knowledge

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F16%3A70665" target="_blank" >RIV/60460709:41210/16:70665 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.parepi.2016.04.001" target="_blank" >http://dx.doi.org/10.1016/j.parepi.2016.04.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.parepi.2016.04.001" target="_blank" >10.1016/j.parepi.2016.04.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Purification of a Fe-SOD excreted byLeishmania braziliensisfor specific antibodies detection in Mexican human sera:Cutting-edge the knowledge

Popis výsledku v původním jazyce

Clinical diagnosis of leishmaniasis is highly complex, presenting a wide range of clinical manifestations, sometimes non-specific, and thus the epidemiological study and diagnostic need specific molecular markers for each Leishmania species. Leishmania spp. posses different Fe-SOD isoforms, one of which is excreted into the external milieu and, presenting immunogenic characteristics, is a very reliable molecular marker. Superoxide dismutases (SODs) are antioxidant metal-enzymes responsible for the dismutation of superoxide ion into hydrogen peroxide and molecular oxygen, and it is considered an important virulence factor. In this manuscript we have purified the iron(Fe)-SOD excreted by Leishmania braziliensis using ion-exchange and molecular-sieve chromatography and we have studied it as an antigen in serodiagnostic analyses in ELISA and Western blot techniques, testing 213 human sera from Mexico. Indeed, L. braziliensis Fe-SODe has been purified 123,26 times with a specific activity of about 893,66

Název v anglickém jazyce

Purification of a Fe-SOD excreted byLeishmania braziliensisfor specific antibodies detection in Mexican human sera:Cutting-edge the knowledge

Popis výsledku anglicky

Clinical diagnosis of leishmaniasis is highly complex, presenting a wide range of clinical manifestations, sometimes non-specific, and thus the epidemiological study and diagnostic need specific molecular markers for each Leishmania species. Leishmania spp. posses different Fe-SOD isoforms, one of which is excreted into the external milieu and, presenting immunogenic characteristics, is a very reliable molecular marker. Superoxide dismutases (SODs) are antioxidant metal-enzymes responsible for the dismutation of superoxide ion into hydrogen peroxide and molecular oxygen, and it is considered an important virulence factor. In this manuscript we have purified the iron(Fe)-SOD excreted by Leishmania braziliensis using ion-exchange and molecular-sieve chromatography and we have studied it as an antigen in serodiagnostic analyses in ELISA and Western blot techniques, testing 213 human sera from Mexico. Indeed, L. braziliensis Fe-SODe has been purified 123,26 times with a specific activity of about 893,66

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasite Epidemiology and Control

ISSN

2405-6731

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NN -

Počet stran výsledku

8

Strana od-do

90-97

Kód UT WoS článku

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EID výsledku v databázi Scopus

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