Quantitative detection of four pome fruit viruses in apple trees throughout the year
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F16%3A70898" target="_blank" >RIV/60460709:41210/16:70898 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.14601/Phytopathol_Mediterr-16825" target="_blank" >http://dx.doi.org/10.14601/Phytopathol_Mediterr-16825</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.14601/Phytopathol_Mediterr-16825" target="_blank" >10.14601/Phytopathol_Mediterr-16825</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Quantitative detection of four pome fruit viruses in apple trees throughout the year
Popis výsledku v původním jazyce
A one-step real-time RT-PCR assay (RT-qPCR) with melting curve analysis, using the green fluorescence dye SYBR Green I, was developed to detect and quantify RNA targets from Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV) and Apple chlorotic leaf spot virus (ACLSV) in infected apple trees. Single PCR products of 87 bp (ApMV), 70 bp (ASGV), 104 bp (ASPV) and 148 bp (ACLSV) were obtained, and melting curve analyses revealed distinct melting temperature peaks for each virus. A dilution series using in vitro synthesized transcripts containing the target sequences as standards yielded a reproducible quantitative assay, with a wide dynamic range of detection and low coefficients of variance. The content of selected viruses in apple plant tissues was stable throughout the year, and their accumulation did not significantly change between different plant tissues. The only minor exceptions were for ApMV and ACLSV, in which noticeable differences in their concentrati
Název v anglickém jazyce
Quantitative detection of four pome fruit viruses in apple trees throughout the year
Popis výsledku anglicky
A one-step real-time RT-PCR assay (RT-qPCR) with melting curve analysis, using the green fluorescence dye SYBR Green I, was developed to detect and quantify RNA targets from Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV) and Apple chlorotic leaf spot virus (ACLSV) in infected apple trees. Single PCR products of 87 bp (ApMV), 70 bp (ASGV), 104 bp (ASPV) and 148 bp (ACLSV) were obtained, and melting curve analyses revealed distinct melting temperature peaks for each virus. A dilution series using in vitro synthesized transcripts containing the target sequences as standards yielded a reproducible quantitative assay, with a wide dynamic range of detection and low coefficients of variance. The content of selected viruses in apple plant tissues was stable throughout the year, and their accumulation did not significantly change between different plant tissues. The only minor exceptions were for ApMV and ACLSV, in which noticeable differences in their concentrati
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GF - Choroby, škůdci, plevely a ochrana rostlin
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PHYTOPATHOLOGIA MEDITERRANEA
ISSN
0031-9465
e-ISSN
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Svazek periodika
55
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
18
Strana od-do
207-224
Kód UT WoS článku
000381767900005
EID výsledku v databázi Scopus
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