Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing – preliminary results

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F16%3A71370" target="_blank" >RIV/60460709:41210/16:71370 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/16:10330114

Výsledek na webu

<a href="http://dx.doi.org/10.17221/27/2016-CJAS" target="_blank" >http://dx.doi.org/10.17221/27/2016-CJAS</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/27/2016-CJAS" target="_blank" >10.17221/27/2016-CJAS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing – preliminary results

Popis výsledku v původním jazyce

Soybean lecithin-based extenders represent an alternative to extenders containing egg yolk, but there has been discussion about their cryoprotective efficacy. Low-density lipoprotein (LDL) was proved as a cryoprotective agent, which can replace egg yolk in extenders. The aim of this study was, at first, to verify quality of produced LDL - newly preserved by sodium azide and especially to investigate the effects of LDL addition on cryoprotective properties of the soybean extenders. The quality of the LDL was verified using commercial egg yolk extender BULLXcell and concentrations of 6%, 8% and 10% LDL (v/v) as an egg yolk replacement. The effects of LDL addition to the soybean lecithin-based extenders in concentrations of 4%, 6% and 8% (v/v) were tested using extenders Andromed and Bioxcell. In total, 64 samples from eight bulls were evaluated. Kinematic parameters of spermatozoa, resulted from Computer Assisted Sperm Analysis, and their viability, evaluated by fluorescent technique, were assessed imm

Název v anglickém jazyce

Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing – preliminary results

Popis výsledku anglicky

Soybean lecithin-based extenders represent an alternative to extenders containing egg yolk, but there has been discussion about their cryoprotective efficacy. Low-density lipoprotein (LDL) was proved as a cryoprotective agent, which can replace egg yolk in extenders. The aim of this study was, at first, to verify quality of produced LDL - newly preserved by sodium azide and especially to investigate the effects of LDL addition on cryoprotective properties of the soybean extenders. The quality of the LDL was verified using commercial egg yolk extender BULLXcell and concentrations of 6%, 8% and 10% LDL (v/v) as an egg yolk replacement. The effects of LDL addition to the soybean lecithin-based extenders in concentrations of 4%, 6% and 8% (v/v) were tested using extenders Andromed and Bioxcell. In total, 64 samples from eight bulls were evaluated. Kinematic parameters of spermatozoa, resulted from Computer Assisted Sperm Analysis, and their viability, evaluated by fluorescent technique, were assessed imm

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210109" target="_blank" >QJ1210109: Inovace zpracování ejakulátu býků a technologických postupů výroby inseminačních dávek vedoucí ke zvýšení jejich oplozovací schopnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

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Svazek periodika

61

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

560-567

Kód UT WoS článku

000390941100003

EID výsledku v databázi Scopus

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