Anti-apoptotic properties of carbon monoxide in porcine oocyte during in vitro aging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A74346" target="_blank" >RIV/60460709:41210/17:74346 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.7717/peerj.3876" target="_blank" >http://dx.doi.org/10.7717/peerj.3876</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7717/peerj.3876" target="_blank" >10.7717/peerj.3876</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Anti-apoptotic properties of carbon monoxide in porcine oocyte during in vitro aging

Popis výsledku v původním jazyce

If fertilization of matured oocyte does not occur, unfertilized oocyte undergoes aging, resulting in a time dependent reduction of the oocyte s quality. The aging of porcine oocytes can lead to apoptosis. Carbon monoxide (CO), a signal molecule produced by the heme oxygenase (HO), possesses cytoprotective and anti-apoptotic effects that have been described in somatic cells. However, the effects of CO in oocytes have yet to be investigated. By immunocytochemistry method we detected that both isoforms of heme oxygenase are present in the porcine oocytes. Based on the morphological signs of oocyte aging, it was found that the inhibition of both HO isoforms by Zn protoporphyrin IX leads to an increase in the number of apoptotic oocytes and decrease in the number of intact oocytes during aging. Contrarily, the presence of CO donors significantly decrease the number of apoptotic oocytes while increasing the number of intact oocytes. We also determined that CO donors significantly decrease the caspase3 (CAS

Název v anglickém jazyce

Anti-apoptotic properties of carbon monoxide in porcine oocyte during in vitro aging

Popis výsledku anglicky

If fertilization of matured oocyte does not occur, unfertilized oocyte undergoes aging, resulting in a time dependent reduction of the oocyte s quality. The aging of porcine oocytes can lead to apoptosis. Carbon monoxide (CO), a signal molecule produced by the heme oxygenase (HO), possesses cytoprotective and anti-apoptotic effects that have been described in somatic cells. However, the effects of CO in oocytes have yet to be investigated. By immunocytochemistry method we detected that both isoforms of heme oxygenase are present in the porcine oocytes. Based on the morphological signs of oocyte aging, it was found that the inhibition of both HO isoforms by Zn protoporphyrin IX leads to an increase in the number of apoptotic oocytes and decrease in the number of intact oocytes during aging. Contrarily, the presence of CO donors significantly decrease the number of apoptotic oocytes while increasing the number of intact oocytes. We also determined that CO donors significantly decrease the caspase3 (CAS

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QJ1510138" target="_blank" >QJ1510138: Inovace biotechnologií v reprodukci hospodářských zvířat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PeerJ

ISSN

2167-8359

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

N

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

25

Strana od-do

1-25

Kód UT WoS článku

000412520500006

EID výsledku v databázi Scopus

2-s2.0-85030710158