Reliable molecular differentiation of Trichuris ovis and Trichuris discolor from sheep (Ovis orientalis aries) and roe deer (Capreolus capreolus) and morphological characterisation of their females: morphology does not work sufficiently
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A74610" target="_blank" >RIV/60460709:41210/17:74610 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s00436-017-5524-9" target="_blank" >http://dx.doi.org/10.1007/s00436-017-5524-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00436-017-5524-9" target="_blank" >10.1007/s00436-017-5524-9</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Reliable molecular differentiation of Trichuris ovis and Trichuris discolor from sheep (Ovis orientalis aries) and roe deer (Capreolus capreolus) and morphological characterisation of their females: morphology does not work sufficiently
Popis výsledku v původním jazyce
The main aim of the study was to evaluate associations between morphological variability of Trichuris females from sheep and roe deer and their rDNA polymorphism in whipworm populations from the Czech Republic. The results introduced the use of new molecular markers based on the internal transcribed spacer (ITS)1 5,8S RNA ITS2 region polymorphisms, as useful tools for the unambiguous differentiation of congeners Trichuris ovis and Trichuris discolor. These markers revealed both parasites in roe deer and in sheep. However, T. ovis females predominated in sheep while T. discolor females occurred mostly in roe deer. Additional analysis of ITS1 5,8 rRNA ITS2 discovered the genetic uniformity of the analysed T. discolor but high haplotype variation of T. ovis. Simultaneously, molecularly designated female individuals of both species were categorised into four morphotypes (MT) on the basis of morphology of genital pore area. MT1 and MT4 (vulvar opening on everted vaginal appendage on visib
Název v anglickém jazyce
Reliable molecular differentiation of Trichuris ovis and Trichuris discolor from sheep (Ovis orientalis aries) and roe deer (Capreolus capreolus) and morphological characterisation of their females: morphology does not work sufficiently
Popis výsledku anglicky
The main aim of the study was to evaluate associations between morphological variability of Trichuris females from sheep and roe deer and their rDNA polymorphism in whipworm populations from the Czech Republic. The results introduced the use of new molecular markers based on the internal transcribed spacer (ITS)1 5,8S RNA ITS2 region polymorphisms, as useful tools for the unambiguous differentiation of congeners Trichuris ovis and Trichuris discolor. These markers revealed both parasites in roe deer and in sheep. However, T. ovis females predominated in sheep while T. discolor females occurred mostly in roe deer. Additional analysis of ITS1 5,8 rRNA ITS2 discovered the genetic uniformity of the analysed T. discolor but high haplotype variation of T. ovis. Simultaneously, molecularly designated female individuals of both species were categorised into four morphotypes (MT) on the basis of morphology of genital pore area. MT1 and MT4 (vulvar opening on everted vaginal appendage on visib
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
<a href="/cs/project/QJ1510038" target="_blank" >QJ1510038: Využití rostlinných extraktů jako bioantiparazitik u hospodářských zvířat</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Parasitology Research
ISSN
0932-0113
e-ISSN
—
Svazek periodika
116
Číslo periodika v rámci svazku
8
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
12
Strana od-do
2199-2210
Kód UT WoS článku
000406423300016
EID výsledku v databázi Scopus
2-s2.0-85020554696