Morphological, physiological and genomic comparisons between diploids and induced tetraploids in Anemone sylvestris L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A75951" target="_blank" >RIV/60460709:41210/18:75951 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41330/18:75951 RIV/60460709:41340/18:75951 RIV/00027073:_____/18:N0000097

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11240-017-1331-3" target="_blank" >http://dx.doi.org/10.1007/s11240-017-1331-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-017-1331-3" target="_blank" >10.1007/s11240-017-1331-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Morphological, physiological and genomic comparisons between diploids and induced tetraploids in Anemone sylvestris L.

Popis výsledku v původním jazyce

The objective of this research was to induce mitotic chromosome doubling in Anemone sylvestris L. The mitosis inhibitor oryzalin was directly added to the induction medium at 1, 2, 5, 10 and 15 uM for 8, 10 or 12 weeks of cultivation. Three tetraploid plants (2n = 4x = 32), 0,8 percent (polyploidization efficiency), were obtained from diploid plants (2n = 2x = 16) in three treatments (1 uM for 10 weeks, 5 uM for 8 weeks and 8 uM for 10 weeks). Ploidy level was confirmed by flow cytometry. Morphological characteristics (e.g. flower diameter, total plant height, leaf area) and chlorophyll content differences between diploid and tetraploid A. sylvestris were observed together with polyphenol content and antioxidant activity. The inter primer binding sites markers were used for evaluation of polymorphism. New genotypes with different morphological and biological characteristics were obtained through somatic polyploidization. The tetraploid plants were stronger, more vigorous and had an early flowering, w

Název v anglickém jazyce

Morphological, physiological and genomic comparisons between diploids and induced tetraploids in Anemone sylvestris L.

Popis výsledku anglicky

The objective of this research was to induce mitotic chromosome doubling in Anemone sylvestris L. The mitosis inhibitor oryzalin was directly added to the induction medium at 1, 2, 5, 10 and 15 uM for 8, 10 or 12 weeks of cultivation. Three tetraploid plants (2n = 4x = 32), 0,8 percent (polyploidization efficiency), were obtained from diploid plants (2n = 2x = 16) in three treatments (1 uM for 10 weeks, 5 uM for 8 weeks and 8 uM for 10 weeks). Ploidy level was confirmed by flow cytometry. Morphological characteristics (e.g. flower diameter, total plant height, leaf area) and chlorophyll content differences between diploid and tetraploid A. sylvestris were observed together with polyphenol content and antioxidant activity. The inter primer binding sites markers were used for evaluation of polymorphism. New genotypes with different morphological and biological characteristics were obtained through somatic polyploidization. The tetraploid plants were stronger, more vigorous and had an early flowering, w

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/GJ17-25536Y" target="_blank" >GJ17-25536Y: Fytoremediace kontaminovaných půd s využitím nanočástic: Implikace pro rhizosféru</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

1573-5044

Svazek periodika

132

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

317-327

Kód UT WoS článku

000422790600007

EID výsledku v databázi Scopus

2-s2.0-85032945336