G protein-coupled estrogen receptor (GPER) in adult boar testes, epididymis and spermatozoa during epididymal maturation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A76550" target="_blank" >RIV/60460709:41210/18:76550 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/18:00492930

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2018.05.015" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2018.05.015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2018.05.015" target="_blank" >10.1016/j.ijbiomac.2018.05.015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

G protein-coupled estrogen receptor (GPER) in adult boar testes, epididymis and spermatozoa during epididymal maturation

Popis výsledku v původním jazyce

The G protein coupled estrogen receptor (GPER) is a transmembrane receptor considered as a mediator of rapid nongenomic responses. GPER has been found in the male reproductive tract of many mammalian species. However, in adult boars, GPER has been reported only in ejaculated spermatozoa. Therefore, we focused on GPER detection in testicular and epididymal tissues and sperm cells in adult boars. We found GPER in Leydig cells and seminiferous tubules of boar testes and in the secretory epithelium of epididymis. A weaker signal was visible in smooth muscle cells and spermatozoa in the epididymal tubule. In spermatozoa isolated from epididymal parts, GPER was found to localize mainly in the spermacrosomeand flagellum.Weimmunodetected several protein bands in the extracts of the tissues and epididymal spermatozoa. A significantly higher amount of GPER mRNA was detected in the spermatozoa fromcaput epididymis, whereas the mRNA expression was lower in tissues of testes and caput epididymal. Our res

Název v anglickém jazyce

G protein-coupled estrogen receptor (GPER) in adult boar testes, epididymis and spermatozoa during epididymal maturation

Popis výsledku anglicky

The G protein coupled estrogen receptor (GPER) is a transmembrane receptor considered as a mediator of rapid nongenomic responses. GPER has been found in the male reproductive tract of many mammalian species. However, in adult boars, GPER has been reported only in ejaculated spermatozoa. Therefore, we focused on GPER detection in testicular and epididymal tissues and sperm cells in adult boars. We found GPER in Leydig cells and seminiferous tubules of boar testes and in the secretory epithelium of epididymis. A weaker signal was visible in smooth muscle cells and spermatozoa in the epididymal tubule. In spermatozoa isolated from epididymal parts, GPER was found to localize mainly in the spermacrosomeand flagellum.Weimmunodetected several protein bands in the extracts of the tissues and epididymal spermatozoa. A significantly higher amount of GPER mRNA was detected in the spermatozoa fromcaput epididymis, whereas the mRNA expression was lower in tissues of testes and caput epididymal. Our res

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnologické a biomedicínské centrum Akademie věd a Univerzity Karlovy</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ISSN

0141-8130

e-ISSN

1879-0003

Svazek periodika

116

Číslo periodika v rámci svazku

N

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

113-119

Kód UT WoS článku

000440120500015

EID výsledku v databázi Scopus

2-s2.0-85047084898