New Insight into Sperm Capacitation: A Novel Mechanism of 17ß-Estradiol Signalling
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A77579" target="_blank" >RIV/60460709:41210/18:77579 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.3390/ijms19124011" target="_blank" >http://dx.doi.org/10.3390/ijms19124011</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms19124011" target="_blank" >10.3390/ijms19124011</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
New Insight into Sperm Capacitation: A Novel Mechanism of 17ß-Estradiol Signalling
Popis výsledku v původním jazyce
Estradiol is a natural estrogen regulating reproduction including sperm and egg development, sperm maturation, called capacitation, and sperm egg communication. High doses can increase germ cell apoptosis and decrease sperm count. Our aim was to answer the biological relevance of estradiol in sperm capacitation and its effect on motility and acrosome reaction to quantify its interaction with estrogen receptors and propose a model of estradiol action during capacitation using kinetic analysis. Estradiol increased protein tyrosine phosphorylation, elevated rate of spontaneous acrosome reaction, and altered motility parameters measured HamiltonThorne Computer Assisted Semen Analyzer (CASA) in capacitating sperm. To monitor time and concentration dependent binding dynamics of extracellular estradiol, high-performance liquid chromatography with tandem mass spectrometry was used to measure sperm response and data was subjected to kinetic analysis. The kinetic model of estradiol action during sperm maturati
Název v anglickém jazyce
New Insight into Sperm Capacitation: A Novel Mechanism of 17ß-Estradiol Signalling
Popis výsledku anglicky
Estradiol is a natural estrogen regulating reproduction including sperm and egg development, sperm maturation, called capacitation, and sperm egg communication. High doses can increase germ cell apoptosis and decrease sperm count. Our aim was to answer the biological relevance of estradiol in sperm capacitation and its effect on motility and acrosome reaction to quantify its interaction with estrogen receptors and propose a model of estradiol action during capacitation using kinetic analysis. Estradiol increased protein tyrosine phosphorylation, elevated rate of spontaneous acrosome reaction, and altered motility parameters measured HamiltonThorne Computer Assisted Semen Analyzer (CASA) in capacitating sperm. To monitor time and concentration dependent binding dynamics of extracellular estradiol, high-performance liquid chromatography with tandem mass spectrometry was used to measure sperm response and data was subjected to kinetic analysis. The kinetic model of estradiol action during sperm maturati
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10604 - Reproductive biology (medical aspects to be 3)
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Svazek periodika
19
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
19
Strana od-do
1-19
Kód UT WoS článku
000455323500313
EID výsledku v databázi Scopus
2-s2.0-85058764880