Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A78011" target="_blank" >RIV/60460709:41210/18:78011 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/18:00506670

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00441-017-2705-x" target="_blank" >http://dx.doi.org/10.1007/s00441-017-2705-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00441-017-2705-x" target="_blank" >10.1007/s00441-017-2705-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis

Popis výsledku v původním jazyce

Phosphorylation, or dephosphorylation, is one of the most frequent posttranslational modifications regulating protein protein activity in eukaryotic cells. Whereas mature spermatozoa (as specialized cells) are transcriptionally inactive and do not synthesize new proteins, phosphorylation of sperm proteins is very important for the regulation of the sperm function. Although the posttesticular maturation of spermatozoa is a process common to all mammals, comparative studies showed significant differences in sperm surface proteins and the mechanisms of protein modification during the epididymal maturation. In our study, the evaluation of tyrosine phosphorylation, represented by the fluorescent patterns of used antiphosphotyrosine antibodies (P Tyr 01 and 4G10), in spermatozoa isolated from different regions of the epididymis caput, corpus and cauda was performed. Although in general both antibodies detected almost the same reaction patterns, we observed some dissimilarity associated with the binding spe

Název v anglickém jazyce

Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis

Popis výsledku anglicky

Phosphorylation, or dephosphorylation, is one of the most frequent posttranslational modifications regulating protein protein activity in eukaryotic cells. Whereas mature spermatozoa (as specialized cells) are transcriptionally inactive and do not synthesize new proteins, phosphorylation of sperm proteins is very important for the regulation of the sperm function. Although the posttesticular maturation of spermatozoa is a process common to all mammals, comparative studies showed significant differences in sperm surface proteins and the mechanisms of protein modification during the epididymal maturation. In our study, the evaluation of tyrosine phosphorylation, represented by the fluorescent patterns of used antiphosphotyrosine antibodies (P Tyr 01 and 4G10), in spermatozoa isolated from different regions of the epididymis caput, corpus and cauda was performed. Although in general both antibodies detected almost the same reaction patterns, we observed some dissimilarity associated with the binding spe

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

<a href="/cs/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnologické a biomedicínské centrum Akademie věd a Univerzity Karlovy</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CELL AND TISSUE RESEARCH

ISSN

0302-766X

e-ISSN

1432-0878

Svazek periodika

371

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

365-373

Kód UT WoS článku

000423324400013

EID výsledku v databázi Scopus

2-s2.0-85031929786