Sample handling and pretreatment as critical points in determining the quality of analytical data during metallothionein determination in wild animals

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3A77282" target="_blank" >RIV/60460709:41210/19:77282 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388955:_____/19:00499050

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1470160X18308641?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1470160X18308641?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ecolind.2018.11.002" target="_blank" >10.1016/j.ecolind.2018.11.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sample handling and pretreatment as critical points in determining the quality of analytical data during metallothionein determination in wild animals

Popis výsledku v původním jazyce

Metallothioneins (MTs) are considered possible markers of environmental pollution, but pre-analysis handling of the tissues in which they are contained can affect their degradation. To determine MT synthesis in wild animals for the purpose of evaluating environmental pollution, the stability of MTs during animal trapping, during the transporting of samples to the laboratory, and during the storing of samples before analysis should be assessed. Five methods of pretreatment processing were tested on 12 male rats under laboratory conditions. MT concentrations were determined by differential pulse voltammetry (DPV) of the modified Brdička reaction. The results show that freeze-drying does not affect the concentration of MTs in the tissue. However, the simulation of extreme field conditions resulted in a significantly lower concentration of MTs in analysed rat liver samples compared with normal conditions. Such samples could be excluded from further evaluation due to differences in the voltammetric record

Název v anglickém jazyce

Sample handling and pretreatment as critical points in determining the quality of analytical data during metallothionein determination in wild animals

Popis výsledku anglicky

Metallothioneins (MTs) are considered possible markers of environmental pollution, but pre-analysis handling of the tissues in which they are contained can affect their degradation. To determine MT synthesis in wild animals for the purpose of evaluating environmental pollution, the stability of MTs during animal trapping, during the transporting of samples to the laboratory, and during the storing of samples before analysis should be assessed. Five methods of pretreatment processing were tested on 12 male rats under laboratory conditions. MT concentrations were determined by differential pulse voltammetry (DPV) of the modified Brdička reaction. The results show that freeze-drying does not affect the concentration of MTs in the tissue. However, the simulation of extreme field conditions resulted in a significantly lower concentration of MTs in analysed rat liver samples compared with normal conditions. Such samples could be excluded from further evaluation due to differences in the voltammetric record

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ECOLOGICAL INDICATORS

ISSN

1470-160X

e-ISSN

1872-7034

Svazek periodika

98

Číslo periodika v rámci svazku

N

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

214-217

Kód UT WoS článku

000464891100023

EID výsledku v databázi Scopus

2-s2.0-85056176740