High mobility group box 1 and TLR4 signaling pathway in gnotobiotic piglets colonized/infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. typhimurium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3AN0000141" target="_blank" >RIV/60460709:41210/19:N0000141 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/19:00519835 RIV/68378050:_____/19:00519835 RIV/67985904:_____/19:00519835

Výsledek na webu

<a href="https://www.mdpi.com/1422-0067/20/24/6294" target="_blank" >https://www.mdpi.com/1422-0067/20/24/6294</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms20246294" target="_blank" >10.3390/ijms20246294</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High mobility group box 1 and TLR4 signaling pathway in gnotobiotic piglets colonized/infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. typhimurium

Popis výsledku v původním jazyce

High mobility group box 1 (HMGB1) is a DNA binding nuclear protein that can be actively secreted by immune cells after different immune stimuli or passively released from cells undergoing necrosis. HMGB1 amplifies inflammation, and its hypersecretion contributes to multiple organ dysfunction syndrome and death. We tested possible immunomodulatory effect of commensal Lactobacillus amylovorus (LA), Lactobacillus mucosae (LM) or probiotic Escherichia coli Nissle 1917 (EcN) in infection of gnotobiotic piglets with Salmonella Typhimurium (ST). Transcription of HMGB1 and Toll like receptors (TLR) 2, 4, and 9 and receptor for advanced glycation end products (RAGE), TLR4 related molecules (MD2, CD14, and LBP), and adaptor proteins (MyD88 and TRIF) in the ileum and colon were measured by RT qPCR. Expression of TLR4 and its related molecules were highly upregulated in the ST infected intestine, which was suppressed by EcN, but not LA nor LM. In contrast, HMGB1 expression was unaffected by ST infection or commensal probiotic administration. HMGB1 protein levels in the intestine measured by ELISA were increased in ST infected piglets, but they were decreased by previous colonization with E. coli Nissle 1917 only. We conclude that the stability of HMGB1 mRNA expression in all piglet groups could show its importance for DNA transcription and physiological cell functions. The presence of HMGB1 protein in the intestinal lumen probably indicates cellular damage.

Název v anglickém jazyce

High mobility group box 1 and TLR4 signaling pathway in gnotobiotic piglets colonized/infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. typhimurium

Popis výsledku anglicky

High mobility group box 1 (HMGB1) is a DNA binding nuclear protein that can be actively secreted by immune cells after different immune stimuli or passively released from cells undergoing necrosis. HMGB1 amplifies inflammation, and its hypersecretion contributes to multiple organ dysfunction syndrome and death. We tested possible immunomodulatory effect of commensal Lactobacillus amylovorus (LA), Lactobacillus mucosae (LM) or probiotic Escherichia coli Nissle 1917 (EcN) in infection of gnotobiotic piglets with Salmonella Typhimurium (ST). Transcription of HMGB1 and Toll like receptors (TLR) 2, 4, and 9 and receptor for advanced glycation end products (RAGE), TLR4 related molecules (MD2, CD14, and LBP), and adaptor proteins (MyD88 and TRIF) in the ileum and colon were measured by RT qPCR. Expression of TLR4 and its related molecules were highly upregulated in the ST infected intestine, which was suppressed by EcN, but not LA nor LM. In contrast, HMGB1 expression was unaffected by ST infection or commensal probiotic administration. HMGB1 protein levels in the intestine measured by ELISA were increased in ST infected piglets, but they were decreased by previous colonization with E. coli Nissle 1917 only. We conclude that the stability of HMGB1 mRNA expression in all piglet groups could show its importance for DNA transcription and physiological cell functions. The presence of HMGB1 protein in the intestinal lumen probably indicates cellular damage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/EF16_019%2F0000845" target="_blank" >EF16_019/0000845: Centrum pro studium vzniku a transformací nutričně významných látek v potravním řetězci v interakci s potenciálně rizikovými látkami antropogenního původu: komplexní posouzení rizika kontaminace půdy pro kvalitu zemědělské produkce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Svazek periodika

20

Číslo periodika v rámci svazku

24

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

1-20

Kód UT WoS článku

000506840100187

EID výsledku v databázi Scopus

2-s2.0-85076856092