Detection of sex in adults and larvae of Leptinotarsa decemlineata on principle of copy number variation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F22%3A91077" target="_blank" >RIV/60460709:41210/22:91077 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60109807:_____/22:N0000003

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-022-08642-x" target="_blank" >https://www.nature.com/articles/s41598-022-08642-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-022-08642-x" target="_blank" >10.1038/s41598-022-08642-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of sex in adults and larvae of Leptinotarsa decemlineata on principle of copy number variation

Popis výsledku v původním jazyce

The identification of sex in larvae of insects is usually challenging or even impossible, while in adults the sexual dimorphism is usually evident. Here, we used copy number analysis to develop a method of sex detection in Colorado potato beetle (Leptinotarsa decemlineata), which has an X0 sex determination system. The X linked gene LdVssc and autosomal gene LdUBE3B were identified as appropriate target and reference loci, respectively. The copy numbers (CNV) of LdVssc in males and females were estimated using standard droplet digital PCR (ddPCR) and realtime PCR (qPCR). With both methods, CNVs were bimodally distributed (BAddPCR = 0,709 and BAqPCR = 0,683) with 100% ability to distinguish females from males. The use of qPCR based sex detection in a broad collection of 448 random CPB adults showed a perfect association (Phi = 1,0, p less 0,05) with the true sexes of adults, with mean CNV in females of 2,032 (SD = 0,227) and 0,989 in males (SD = 0,147). In the collection of 50 random 4th instar larvae

Název v anglickém jazyce

Detection of sex in adults and larvae of Leptinotarsa decemlineata on principle of copy number variation

Popis výsledku anglicky

The identification of sex in larvae of insects is usually challenging or even impossible, while in adults the sexual dimorphism is usually evident. Here, we used copy number analysis to develop a method of sex detection in Colorado potato beetle (Leptinotarsa decemlineata), which has an X0 sex determination system. The X linked gene LdVssc and autosomal gene LdUBE3B were identified as appropriate target and reference loci, respectively. The copy numbers (CNV) of LdVssc in males and females were estimated using standard droplet digital PCR (ddPCR) and realtime PCR (qPCR). With both methods, CNVs were bimodally distributed (BAddPCR = 0,709 and BAqPCR = 0,683) with 100% ability to distinguish females from males. The use of qPCR based sex detection in a broad collection of 448 random CPB adults showed a perfect association (Phi = 1,0, p less 0,05) with the true sexes of adults, with mean CNV in females of 2,032 (SD = 0,227) and 0,989 in males (SD = 0,147). In the collection of 50 random 4th instar larvae

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10620 - Other biological topics

Projekt

<a href="/cs/project/QK1910270" target="_blank" >QK1910270: Inovace integrované ochrany brambor proti mandelince bramborové založené na nových poznatcích genetických a biologických charakteristik</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

0-0

Kód UT WoS článku

000770396200071

EID výsledku v databázi Scopus

2-s2.0-85126553185