Emergence of fungicide sensitivity in Leptosphaeria maculans isolates collected from the Czech Republic to DMI fungicides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F22%3A91450" target="_blank" >RIV/60460709:41210/22:91450 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2077-0472/12/2/237/htm" target="_blank" >https://www.mdpi.com/2077-0472/12/2/237/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agriculture12020237" target="_blank" >10.3390/agriculture12020237</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Emergence of fungicide sensitivity in Leptosphaeria maculans isolates collected from the Czech Republic to DMI fungicides

Popis výsledku v původním jazyce

In the Czech Republic, demethylation inhibitors (DMIs) are used both as fungicides in controlling phoma stem canker and as growth regulators. This heavy use can result in the development of resistant isolates. A total of 45 and 286 Leptosphaeria maculans isolates were tested in vitro, using the mycelial growth and microtiter plate assays, respectively. The objective was to determine the sensitivity of L. maculans isolates collected in the Czech Republic to the fungicides tetraconazole, metconazole, and prochloraz. The mean EC50 values with the mycelial growth plate method were 1,33, 0,78, and 0,40 microgram/mL for tetraconazole, metconazole, and prochloraz, respectively. The mean EC50 values for the microtiter plate assay were 3,01, 0,44, and 0,19 microgram/mL for tetraconazole, metconazole, and prochloraz, respectively. All three fungicides also had high variation factors that may be due to inserts in the ERG11 promoter region. In addition, cross sensitivity among the three fungicides was observed.

Název v anglickém jazyce

Emergence of fungicide sensitivity in Leptosphaeria maculans isolates collected from the Czech Republic to DMI fungicides

Popis výsledku anglicky

In the Czech Republic, demethylation inhibitors (DMIs) are used both as fungicides in controlling phoma stem canker and as growth regulators. This heavy use can result in the development of resistant isolates. A total of 45 and 286 Leptosphaeria maculans isolates were tested in vitro, using the mycelial growth and microtiter plate assays, respectively. The objective was to determine the sensitivity of L. maculans isolates collected in the Czech Republic to the fungicides tetraconazole, metconazole, and prochloraz. The mean EC50 values with the mycelial growth plate method were 1,33, 0,78, and 0,40 microgram/mL for tetraconazole, metconazole, and prochloraz, respectively. The mean EC50 values for the microtiter plate assay were 3,01, 0,44, and 0,19 microgram/mL for tetraconazole, metconazole, and prochloraz, respectively. All three fungicides also had high variation factors that may be due to inserts in the ERG11 promoter region. In addition, cross sensitivity among the three fungicides was observed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agriculture-BASEL

ISSN

2077-0472

e-ISSN

2077-0472

Svazek periodika

12

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

0-0

Kód UT WoS článku

000763288000001

EID výsledku v databázi Scopus

2-s2.0-85124400909