Effect of extender on the quality and incubation resilience of cryopreserved Holstein bull semen

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F22%3A91762" target="_blank" >RIV/60460709:41210/22:91762 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.agriculturejournals.cz/web/cjas.htm?type=article&id=196_2021-CJAS" target="_blank" >https://www.agriculturejournals.cz/web/cjas.htm?type=article&id=196_2021-CJAS</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/196/2021-CJAS" target="_blank" >10.17221/196/2021-CJAS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of extender on the quality and incubation resilience of cryopreserved Holstein bull semen

Popis výsledku v původním jazyce

There are still concerns over sperm quality when plant-based diluents are used instead of animal-based semen extenders. Therefore, in our study we compared the effects of one soybean lecithin-based (AndroMed) and two egg yolk-based (BullXcell and Optidyl) commercially available extenders on post-thaw in vitro sperm quality. Fifty ejaculates collected from ten bulls were aliquoted into three parts and diluted with the above-mentioned extenders. Post-thaw sperm viability, mitochondrial membrane potential (MMP), plasma membrane integrity, and acrosome status were analysed immediately after thawing (0 h) and at an hourly interval during two hours incubation at 38 degrees of C. Sperm functionality variables were assessed by simultaneous quadruple staining using flow cytometry. Semen stored in Optidyl had a greater viability, plasma membrane and acrosome integrity than that stored in AndroMed and BullXcell. With the use of BullXcell there was a higher percentage of sperm with high MMP when compared with th

Název v anglickém jazyce

Effect of extender on the quality and incubation resilience of cryopreserved Holstein bull semen

Popis výsledku anglicky

There are still concerns over sperm quality when plant-based diluents are used instead of animal-based semen extenders. Therefore, in our study we compared the effects of one soybean lecithin-based (AndroMed) and two egg yolk-based (BullXcell and Optidyl) commercially available extenders on post-thaw in vitro sperm quality. Fifty ejaculates collected from ten bulls were aliquoted into three parts and diluted with the above-mentioned extenders. Post-thaw sperm viability, mitochondrial membrane potential (MMP), plasma membrane integrity, and acrosome status were analysed immediately after thawing (0 h) and at an hourly interval during two hours incubation at 38 degrees of C. Sperm functionality variables were assessed by simultaneous quadruple staining using flow cytometry. Semen stored in Optidyl had a greater viability, plasma membrane and acrosome integrity than that stored in AndroMed and BullXcell. With the use of BullXcell there was a higher percentage of sperm with high MMP when compared with th

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910242" target="_blank" >QK1910242: Eliminace rizikových faktorů zdraví a reprodukce dojnic pomocí využití automatizovaných systémů měření a sběru dat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

1805-9309

Svazek periodika

67

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

12

Strana od-do

75-86

Kód UT WoS článku

000760811100001

EID výsledku v databázi Scopus

2-s2.0-85128870142