Schistosoma mansoni MEG family proteins in the environment of host-parasite interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F22%3AN0000022" target="_blank" >RIV/60460709:41210/22:N0000022 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Schistosoma mansoni MEG family proteins in the environment of host-parasite interactions

Popis výsledku v původním jazyce

Schistosomiasis is a vectorborne parasitic disease affecting over 250 million people in Africa, the Caribbean, South America, SouthEast Asia and Mediterranean Europe, it is one of the most devastating parasitic diseases worldwide. In the egg secretome of Schistosoma mansoni, about 188 proteins have been identified, of which only a few have been well characterized. One group of highly expressed proteins of the S. mansoni egg secretome is represented by an enigmatic group of genes referred to as MEGs (Micro-Exon Genes). S. mansoni MEGs contain short symmetric exons comprising about 80 percent of the entire gene sequence. Proteins encoded by this group of genes represent a unique system of protein variants generated by alternative splicing. Our lab identified three MEG members in the egg transcriptome, yet they belong to the most highly expressed proteins in mature eggs. Based on reported interactions between S. mansoni eggs and their human host, transcriptomic analyses and already performed experiments, we suggest that these highly expressed MEG proteins and their splice variants could play an essential role in host-parasite interactions. This project aims to use a combination of computational and experimental work to determine their structure and interaction partners. In silico methods used to achieve this objective are ab inito structure prediction r homology modelling, interaction studies will be performed using extracellular matrix interaction partner prediction (MatrixDB) or molecular docking. These methods are accompanied by the expression of S. mansoni MEG proteins and their biophysical analysis (CD, DLS, SAXS) and subsequent structure determination by NMR. 21st European Conference on Computational Biology 12-21 September 2022 Sitges, Barcelona. Molecular interactome of the parasite Schistosoma mansoni and its human host (35/2021) financed from the OP RDE project Improvement in Qality of the Internal Grant Scheme at CZU, reg. no. CZ.02.2.69/0.0/0.0/19_073/0016944 Czech Ministry of Education (Grant No. LTAUSA19).

Název v anglickém jazyce

Schistosoma mansoni MEG family proteins in the environment of host-parasite interactions

Popis výsledku anglicky

Schistosomiasis is a vectorborne parasitic disease affecting over 250 million people in Africa, the Caribbean, South America, SouthEast Asia and Mediterranean Europe, it is one of the most devastating parasitic diseases worldwide. In the egg secretome of Schistosoma mansoni, about 188 proteins have been identified, of which only a few have been well characterized. One group of highly expressed proteins of the S. mansoni egg secretome is represented by an enigmatic group of genes referred to as MEGs (Micro-Exon Genes). S. mansoni MEGs contain short symmetric exons comprising about 80 percent of the entire gene sequence. Proteins encoded by this group of genes represent a unique system of protein variants generated by alternative splicing. Our lab identified three MEG members in the egg transcriptome, yet they belong to the most highly expressed proteins in mature eggs. Based on reported interactions between S. mansoni eggs and their human host, transcriptomic analyses and already performed experiments, we suggest that these highly expressed MEG proteins and their splice variants could play an essential role in host-parasite interactions. This project aims to use a combination of computational and experimental work to determine their structure and interaction partners. In silico methods used to achieve this objective are ab inito structure prediction r homology modelling, interaction studies will be performed using extracellular matrix interaction partner prediction (MatrixDB) or molecular docking. These methods are accompanied by the expression of S. mansoni MEG proteins and their biophysical analysis (CD, DLS, SAXS) and subsequent structure determination by NMR. 21st European Conference on Computational Biology 12-21 September 2022 Sitges, Barcelona. Molecular interactome of the parasite Schistosoma mansoni and its human host (35/2021) financed from the OP RDE project Improvement in Qality of the Internal Grant Scheme at CZU, reg. no. CZ.02.2.69/0.0/0.0/19_073/0016944 Czech Ministry of Education (Grant No. LTAUSA19).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30310 - Parasitology

Projekt

<a href="/cs/project/EF19_073%2F0016944" target="_blank" >EF19_073/0016944: Zvyšování kvality interního grantového schématu na ČZU</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů