Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A94542" target="_blank" >RIV/60460709:41210/23:94542 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2076-2615/13/7/1200" target="_blank" >https://www.mdpi.com/2076-2615/13/7/1200</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani13071200" target="_blank" >10.3390/ani13071200</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

Popis výsledku v původním jazyce

This study investigated the effect of cryoprotectant glycerol addition in different phases of sperm equilibration on sperm motility and viability parameters of thawed ram spermatozoa. The purpose of this study was to compare two strategies of glycerol addition for sperm cryo-preservation. The first strategy included the use of a glycerol-free extender for the procedure of glycerol-free equilibration and chilling, with the glycerolation of the extender by 6% glycerol shortly before sperm slow freezing. The second strategy included the use of a freezing extender already glycerolated by 6% glycerol before the equilibration and chilling of sperm and following slow freezing. The obtained results were statistically significant and demonstrated that the glycerol-free equilibration with the addition of glycerol at least 10 min before freezing is the best strategy for cryopreservation of Wallachian ram sperm. This represents a perspective strategy in the cryopreservation process for local sheep breeds and indicates a potential strategy to test for ram cryopreservation in a generalized manner.

Název v anglickém jazyce

Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

Popis výsledku anglicky

This study investigated the effect of cryoprotectant glycerol addition in different phases of sperm equilibration on sperm motility and viability parameters of thawed ram spermatozoa. The purpose of this study was to compare two strategies of glycerol addition for sperm cryo-preservation. The first strategy included the use of a glycerol-free extender for the procedure of glycerol-free equilibration and chilling, with the glycerolation of the extender by 6% glycerol shortly before sperm slow freezing. The second strategy included the use of a freezing extender already glycerolated by 6% glycerol before the equilibration and chilling of sperm and following slow freezing. The obtained results were statistically significant and demonstrated that the glycerol-free equilibration with the addition of glycerol at least 10 min before freezing is the best strategy for cryopreservation of Wallachian ram sperm. This represents a perspective strategy in the cryopreservation process for local sheep breeds and indicates a potential strategy to test for ram cryopreservation in a generalized manner.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animals

ISSN

2076-2615

e-ISSN

2076-2615

Svazek periodika

13

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

1-11

Kód UT WoS článku

000973094100001

EID výsledku v databázi Scopus

2-s2.0-85153246497