Systematic Identification of Suitable Reference Genes for Quantitative Real-Time PCR Analysis in Melissa officinalis L

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A94778" target="_blank" >RIV/60460709:41210/23:94778 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41340/23:94778

Výsledek na webu

<a href="https://www.mdpi.com/2223-7747/12/3/470" target="_blank" >https://www.mdpi.com/2223-7747/12/3/470</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/plants12030470" target="_blank" >10.3390/plants12030470</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Systematic Identification of Suitable Reference Genes for Quantitative Real-Time PCR Analysis in Melissa officinalis L

Popis výsledku v původním jazyce

Melissa officinalis L. is well known for its lemon scented aroma and various pharmacological properties. Despite these valuable properties, the genes involved in the biosynthetic pathways in M. officinalis are not yet well explored when compared to other members of the mint family. For that, gene expression studies using quantitative real time PCR are an excellent tool. Although qRT PCR can provide accurate results, its accuracy is highly reliant on the expression and stability of the reference gene used for normalization. Hence, selecting a suitable experiment specific reference gene is very crucial to obtain accurate results. However, to date, there are no reports for experiment specific reference genes in M. officinalis. Therefore, in the current study, ten commonly used reference genes were assessed for their suitability as optimal reference genes in M. officinalis under various abiotic stress conditions and different plant organs. The candidate genes were ranked based on BestKeeper, comparative Ct, geNorm, NormFinder, and RefFinder. Based on the results, we recommend the combination of EF and GAPDH as the best reference genes to normalize gene expression studies in M. officinalis. On the contrary, HLH71 was identified as the least performing gene. Thereafter, the reliability of the optimal gene combination was assessed by evaluating the relative gene expression of the phenylalanine ammonia lyase gene under two elicitor treatments. PAL is a crucial gene involved directly or indirectly in the production of various economically important secondary metabolites in plants. Suitable reference genes for each experimental condition are also discussed. The findings of the current study form a basis for current and future gene expression studies in M. officinalis and other related species.

Název v anglickém jazyce

Systematic Identification of Suitable Reference Genes for Quantitative Real-Time PCR Analysis in Melissa officinalis L

Popis výsledku anglicky

Melissa officinalis L. is well known for its lemon scented aroma and various pharmacological properties. Despite these valuable properties, the genes involved in the biosynthetic pathways in M. officinalis are not yet well explored when compared to other members of the mint family. For that, gene expression studies using quantitative real time PCR are an excellent tool. Although qRT PCR can provide accurate results, its accuracy is highly reliant on the expression and stability of the reference gene used for normalization. Hence, selecting a suitable experiment specific reference gene is very crucial to obtain accurate results. However, to date, there are no reports for experiment specific reference genes in M. officinalis. Therefore, in the current study, ten commonly used reference genes were assessed for their suitability as optimal reference genes in M. officinalis under various abiotic stress conditions and different plant organs. The candidate genes were ranked based on BestKeeper, comparative Ct, geNorm, NormFinder, and RefFinder. Based on the results, we recommend the combination of EF and GAPDH as the best reference genes to normalize gene expression studies in M. officinalis. On the contrary, HLH71 was identified as the least performing gene. Thereafter, the reliability of the optimal gene combination was assessed by evaluating the relative gene expression of the phenylalanine ammonia lyase gene under two elicitor treatments. PAL is a crucial gene involved directly or indirectly in the production of various economically important secondary metabolites in plants. Suitable reference genes for each experimental condition are also discussed. The findings of the current study form a basis for current and future gene expression studies in M. officinalis and other related species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plants-BASEL

ISSN

2223-7747

e-ISSN

2223-7747

Svazek periodika

12

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

—

Kód UT WoS článku

000930208300001

EID výsledku v databázi Scopus

2-s2.0-85147869381