Genomic characterization and population structure of Croatian Arabian horse

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A96217" target="_blank" >RIV/60460709:41210/23:96217 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.livsci.2023.105343" target="_blank" >https://doi.org/10.1016/j.livsci.2023.105343</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.livsci.2023.105343" target="_blank" >10.1016/j.livsci.2023.105343</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genomic characterization and population structure of Croatian Arabian horse

Popis výsledku v původním jazyce

The long history of Arabian horse breeding in Croatia spans several centuries and was strongly influenced by the breeding of Arabian horses in Bosnia and Herzegovina. Thus, the modern breeding of the Croatian Arabian horse is closely connected with the horse breeding of the Borike Stud, founded in 1895 in the mountainous region of Bosnia. Our main goal was to study the specifics of the Croatian Arabian horse (ARABCRO), the influence of the “Borike” breeding and the possible influence of other horse breeds. Therefore, we analyzed the population structure and admixture of the ARABCRO population (62 horses) together with 538 horses of Arabian and other origin (530 publicly available genotypes and 8 newly genotyped Shagya horses from Croatia) using high-throughput genomic data (GeneSeek® Genomic Profiler™ Equine SNP BeadChip 70 K). Our analyses (PCA, Neighbour Network, FST and STRUCTURE) revealed that ARABCRO is genetically closely related to the other Arabian horse breeds (populations) with an average FST of 0.09 whereas (the FST between ARABCRO and the Kladrub, Lipizzaner or Thoroughbred breeds, for example, is over 0.14) and thus can be considered a member of a large “Arabian horse metapopulation” (ARABMETA). At the same time, we have shown that ARABCRO is a distinct population with a specific position within ARABMETA, which can be quantified by genomic analyses. We also estimated the conservation status of ARABCRO, i.e., genomic inbreeding level (FROH) and effective population size (Ne) given the small census size of the breed (428 horses in 2021). Estimated genomic inbreeding levels were high (FROH>2Mb = 0.136, FROH>4Mb = 0.100), especially those estimated for recent inbreeding (FROH>8Mb = 0.071 and FROH>16Mb = 0.039), indicating intentional mating practices of very close relatives. The estimated effective population size was small, ranging from Ne = 47 to Ne = 67 (95% CI) and was significantly reduced from Ne = 500 (approximately) from 9 to 12 generations back (generation interval about 10 years). The obtained results will be used in further breeding of ARABCRO, especially with regard to the current conservation status, e.g. by controlled mating and “blood refreshment” with other Arabian horses.

Název v anglickém jazyce

Genomic characterization and population structure of Croatian Arabian horse

Popis výsledku anglicky

The long history of Arabian horse breeding in Croatia spans several centuries and was strongly influenced by the breeding of Arabian horses in Bosnia and Herzegovina. Thus, the modern breeding of the Croatian Arabian horse is closely connected with the horse breeding of the Borike Stud, founded in 1895 in the mountainous region of Bosnia. Our main goal was to study the specifics of the Croatian Arabian horse (ARABCRO), the influence of the “Borike” breeding and the possible influence of other horse breeds. Therefore, we analyzed the population structure and admixture of the ARABCRO population (62 horses) together with 538 horses of Arabian and other origin (530 publicly available genotypes and 8 newly genotyped Shagya horses from Croatia) using high-throughput genomic data (GeneSeek® Genomic Profiler™ Equine SNP BeadChip 70 K). Our analyses (PCA, Neighbour Network, FST and STRUCTURE) revealed that ARABCRO is genetically closely related to the other Arabian horse breeds (populations) with an average FST of 0.09 whereas (the FST between ARABCRO and the Kladrub, Lipizzaner or Thoroughbred breeds, for example, is over 0.14) and thus can be considered a member of a large “Arabian horse metapopulation” (ARABMETA). At the same time, we have shown that ARABCRO is a distinct population with a specific position within ARABMETA, which can be quantified by genomic analyses. We also estimated the conservation status of ARABCRO, i.e., genomic inbreeding level (FROH) and effective population size (Ne) given the small census size of the breed (428 horses in 2021). Estimated genomic inbreeding levels were high (FROH>2Mb = 0.136, FROH>4Mb = 0.100), especially those estimated for recent inbreeding (FROH>8Mb = 0.071 and FROH>16Mb = 0.039), indicating intentional mating practices of very close relatives. The estimated effective population size was small, ranging from Ne = 47 to Ne = 67 (95% CI) and was significantly reduced from Ne = 500 (approximately) from 9 to 12 generations back (generation interval about 10 years). The obtained results will be used in further breeding of ARABCRO, especially with regard to the current conservation status, e.g. by controlled mating and “blood refreshment” with other Arabian horses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910156" target="_blank" >QK1910156: Nové postupy pro záchranu ohrožených populací hospodářských zvířat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Livestock Science

ISSN

1871-1413

e-ISSN

1871-1413

Svazek periodika

277

Číslo periodika v rámci svazku

2023-11-01

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

—

Kód UT WoS článku

001096621600001

EID výsledku v databázi Scopus

2-s2.0-85173701097