Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021–2023
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A96949" target="_blank" >RIV/60460709:41210/23:96949 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.17221/152/2023-CJAS" target="_blank" >https://doi.org/10.17221/152/2023-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/152/2023-CJAS" target="_blank" >10.17221/152/2023-CJAS</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021–2023
Popis výsledku v původním jazyce
Flow cytometry (FC) is a very powerful method for the assessment of spermatozoa quality in multiple livestock species, including bulls. Nowadays, many flow cytometric tests are available for quality detection of bull sperm. These tests are based on fluorochromes (fluorescent tags or dyes) and the use of correct concentrations of fluorochromes is an essential aspect of the successful optimization of experimental methodology. Modern flow cytometers are much more affordable, such that spermatologists are now able to use FC methodology (and even image cytometry) in their studies. The present review aims to give a summary of current flow cytometric methodologies used to assess bull sperm quality by flow cytometry. Namely, we give here the concentrations of fluorochromes used in the studies related to bull sperm quality evaluation by flow cytometry published in the last three years (from 2021 to 2023). Importantly, in the present review, the concentration of fluorochromes is unified and presented using comparable molarity units. Furthermore, the rationale of each flow cytometric methodology for particular fluorescent tests, and the citation for the corresponding original methodology, are given. This review will help spermatologists shorten the time needed for the optimization of their flow cytometry methodology for the assessment of bull sperm quality.
Název v anglickém jazyce
Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021–2023
Popis výsledku anglicky
Flow cytometry (FC) is a very powerful method for the assessment of spermatozoa quality in multiple livestock species, including bulls. Nowadays, many flow cytometric tests are available for quality detection of bull sperm. These tests are based on fluorochromes (fluorescent tags or dyes) and the use of correct concentrations of fluorochromes is an essential aspect of the successful optimization of experimental methodology. Modern flow cytometers are much more affordable, such that spermatologists are now able to use FC methodology (and even image cytometry) in their studies. The present review aims to give a summary of current flow cytometric methodologies used to assess bull sperm quality by flow cytometry. Namely, we give here the concentrations of fluorochromes used in the studies related to bull sperm quality evaluation by flow cytometry published in the last three years (from 2021 to 2023). Importantly, in the present review, the concentration of fluorochromes is unified and presented using comparable molarity units. Furthermore, the rationale of each flow cytometric methodology for particular fluorescent tests, and the citation for the corresponding original methodology, are given. This review will help spermatologists shorten the time needed for the optimization of their flow cytometry methodology for the assessment of bull sperm quality.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40101 - Agriculture
Návaznosti výsledku
Projekt
<a href="/cs/project/QK22010270" target="_blank" >QK22010270: Optimalizace řízení individuální reprodukční výkonnosti dojeného skotu</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
1212-1819
Svazek periodika
68
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
17
Strana od-do
469-485
Kód UT WoS článku
001131885100004
EID výsledku v databázi Scopus
2-s2.0-85181500788