1H NMR metabolomics in honeybee research: individual analysis of honeybee body parts and castes comparison (worker and queen bees)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F24%3A96420" target="_blank" >RIV/60460709:41210/24:96420 - isvavai.cz</a>

Výsledek na webu

<a href="https://euromar2023.org/wp-content/uploads/2023/07/PA-Book-v3.pdf" target="_blank" >https://euromar2023.org/wp-content/uploads/2023/07/PA-Book-v3.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

1H NMR metabolomics in honeybee research: individual analysis of honeybee body parts and castes comparison (worker and queen bees)

Popis výsledku v původním jazyce

Agriculture and human food security sectors heavily rely on honeybees. It is estimated that honeybees pollinate around one-third of the world’s crop production. Metabolomics using NMR spectroscopy provides valuable insight into biological functions that are applied in various scientific disciplines. In honeybee research, NMR metabolomics is a promising diagnostic tool that has yet to be fully explored. The purpose of this study was to employ 1H NMR to understand the distribution of metabolites in the body parts of honeybee workers and queens (head, thorax, abdomen, and gut), as well as the metabolic characteristics between the castes. The samples consisted of pooled bees, nurses, foragers, and queens. Bees were anesthetized with CO2 before processing. Each body part was dissected and collected separately. Homogenized samples were extracted with methanol, reconstituted in D2O, and analyzed using a Bruker Avance III spectrometer with a BBFO probe operating at 500.18 MHz for 1H NMR. The spectra were pre- processed, and the selected spectral regions were integrated. This resulted in 156 buckets, of which 73 were identified using Chenomx and an in-house library. Multivariate analysis of PCA, OPLS-DA, and hierarchical clustering were applied. Each body part of the workers and queens contained metabolites corresponding to their physiological functions. The heads of queens and workers have markedly high concentrations of 10- hydroxy-2-decenoic acid, a royal jelly acid. Notable concentrations of (2E)-9-oxodecenoic acid, a queen pheromone, were found in the queens’ heads. In contrast, workers did not possess any (2E)-9- oxodecenoic acid, whereas 3-hydroxykynurenine levels were significantly high. Among worker bees, nurses’ heads had significantly higher concentrations of glucose and fructose than foragers’ heads. The results of this study suggest the characteristic biomarkers of bee body parts and support the expansion of NMR-based metabolomics studies in honeybee research. This research is supported by the Czech Republic’s Ministry of Agriculture (QK21010088).

Název v anglickém jazyce

1H NMR metabolomics in honeybee research: individual analysis of honeybee body parts and castes comparison (worker and queen bees)

Popis výsledku anglicky

Agriculture and human food security sectors heavily rely on honeybees. It is estimated that honeybees pollinate around one-third of the world’s crop production. Metabolomics using NMR spectroscopy provides valuable insight into biological functions that are applied in various scientific disciplines. In honeybee research, NMR metabolomics is a promising diagnostic tool that has yet to be fully explored. The purpose of this study was to employ 1H NMR to understand the distribution of metabolites in the body parts of honeybee workers and queens (head, thorax, abdomen, and gut), as well as the metabolic characteristics between the castes. The samples consisted of pooled bees, nurses, foragers, and queens. Bees were anesthetized with CO2 before processing. Each body part was dissected and collected separately. Homogenized samples were extracted with methanol, reconstituted in D2O, and analyzed using a Bruker Avance III spectrometer with a BBFO probe operating at 500.18 MHz for 1H NMR. The spectra were pre- processed, and the selected spectral regions were integrated. This resulted in 156 buckets, of which 73 were identified using Chenomx and an in-house library. Multivariate analysis of PCA, OPLS-DA, and hierarchical clustering were applied. Each body part of the workers and queens contained metabolites corresponding to their physiological functions. The heads of queens and workers have markedly high concentrations of 10- hydroxy-2-decenoic acid, a royal jelly acid. Notable concentrations of (2E)-9-oxodecenoic acid, a queen pheromone, were found in the queens’ heads. In contrast, workers did not possess any (2E)-9- oxodecenoic acid, whereas 3-hydroxykynurenine levels were significantly high. Among worker bees, nurses’ heads had significantly higher concentrations of glucose and fructose than foragers’ heads. The results of this study suggest the characteristic biomarkers of bee body parts and support the expansion of NMR-based metabolomics studies in honeybee research. This research is supported by the Czech Republic’s Ministry of Agriculture (QK21010088).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40500 - Other agricultural sciences

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

U - Předmět řešení projektu je utajovanou skutečností podle zvláštních právních předpisů nebo je skutečností, jejíž zveřejnění by mohlo ohrozit činnost zpravodajské služby. Údaje o projektu jsou upraveny tak, aby byly zveřejnitelné