Oryzalin-Induced polyploidy enhances natural metabolite accumulation and functional bioactivities in novel Callisia fragrans genotype

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102090" target="_blank" >RIV/60460709:41210/25:102090 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41340/25:102090

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-025-10588-9" target="_blank" >https://www.nature.com/articles/s41598-025-10588-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-10588-9" target="_blank" >10.1038/s41598-025-10588-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Oryzalin-Induced polyploidy enhances natural metabolite accumulation and functional bioactivities in novel Callisia fragrans genotype

Popis výsledku v původním jazyce

The reorientation of Callisia fragrans (Lindl.) Woodson, from therapeutic to ornamental use, exemplifies a broader domestication trend, favoring aesthetics over medicinal properties. Renewed phytomedicinal interest and the rise of plant extract markets drive demand for organic, sustainable consumer products, heightening the competition for superior cultivars. Synthetic polyploidization using oryzalin was conducted to obtain high-quality cultivars of C. fragrans, facilitating enhanced phenotypic and biological traits without genetic modification. This study aimed to explore the metabolic spectrum and biological activities of oryzalin-induced polyploid C. fragrans for its advanced medicinal application. Flow cytometric analysis confirmed the ploidy level of the plants. Consequently, GC-FID and 1H NMR analyses revealed distinct metabolite profiles, with increased ethyl stearate, malic acid, gallic acid, fumaric acid, and unique compounds like (Z)-11-eicosenoic acid and dodecan-1-ol in polyploids. Polyploid extracts demonstrated exceptional antioxidant capacity, with DPPH, ORAC, and ABTS assays showing higher radical scavenging and oxygen absorbance abilities than diploid extracts. The polyploid extract showed enhanced antimicrobial activity against skin pathogens, including Methicillin-resistant Staphylococcus aureus (MRSA). Callisia extracts, meticulously at a low concentration of 25 mu g/mL, showed cytoprotective effects on HT-29 cells, mitigating H2O2-induced oxidative stress. Furthermore, treatment with polyploid extract was associated with the downregulation of the expression of pro-inflammatory enzymes COX-1 and COX-2, suggesting a potentially greater anti-inflammatory effect compared to the diploid extract. These findings depict enhanced metabolite accumulation and biological activities in polyploid compared than diploid progenitor, highlighting the potential of the novel polyploid C. fragrans variety for future therapeutic applications, particularly in pharmaceutical and cosmetic industries.

Název v anglickém jazyce

Oryzalin-Induced polyploidy enhances natural metabolite accumulation and functional bioactivities in novel Callisia fragrans genotype

Popis výsledku anglicky

The reorientation of Callisia fragrans (Lindl.) Woodson, from therapeutic to ornamental use, exemplifies a broader domestication trend, favoring aesthetics over medicinal properties. Renewed phytomedicinal interest and the rise of plant extract markets drive demand for organic, sustainable consumer products, heightening the competition for superior cultivars. Synthetic polyploidization using oryzalin was conducted to obtain high-quality cultivars of C. fragrans, facilitating enhanced phenotypic and biological traits without genetic modification. This study aimed to explore the metabolic spectrum and biological activities of oryzalin-induced polyploid C. fragrans for its advanced medicinal application. Flow cytometric analysis confirmed the ploidy level of the plants. Consequently, GC-FID and 1H NMR analyses revealed distinct metabolite profiles, with increased ethyl stearate, malic acid, gallic acid, fumaric acid, and unique compounds like (Z)-11-eicosenoic acid and dodecan-1-ol in polyploids. Polyploid extracts demonstrated exceptional antioxidant capacity, with DPPH, ORAC, and ABTS assays showing higher radical scavenging and oxygen absorbance abilities than diploid extracts. The polyploid extract showed enhanced antimicrobial activity against skin pathogens, including Methicillin-resistant Staphylococcus aureus (MRSA). Callisia extracts, meticulously at a low concentration of 25 mu g/mL, showed cytoprotective effects on HT-29 cells, mitigating H2O2-induced oxidative stress. Furthermore, treatment with polyploid extract was associated with the downregulation of the expression of pro-inflammatory enzymes COX-1 and COX-2, suggesting a potentially greater anti-inflammatory effect compared to the diploid extract. These findings depict enhanced metabolite accumulation and biological activities in polyploid compared than diploid progenitor, highlighting the potential of the novel polyploid C. fragrans variety for future therapeutic applications, particularly in pharmaceutical and cosmetic industries.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10700 - Other natural sciences

Projekt

<a href="/cs/project/QK1910209" target="_blank" >QK1910209: Výzkum a vývoj produkce léčivých hub v ČR a jejich inovativní aplikace ve funkčních potravinách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

—

Kód UT WoS článku

001530067200018

EID výsledku v databázi Scopus

2-s2.0-105010748496