Strength of 1H NMR in Plant Science and Biotic Stress Research

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102193" target="_blank" >RIV/60460709:41210/25:102193 - isvavai.cz</a>

Výsledek na webu

<a href="https://drive.google.com/file/d/1CdgEHiWx8tb3rgTngQqbi4dIwaqINLmg/view" target="_blank" >https://drive.google.com/file/d/1CdgEHiWx8tb3rgTngQqbi4dIwaqINLmg/view</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Strength of 1H NMR in Plant Science and Biotic Stress Research

Popis výsledku v původním jazyce

Metabolomics has become an important tool in elucidating the complex relationship between a plant genotype and phenotype. For over 20 years, nuclear magnetic resonance (NMR) spectroscopy has been known for its robustness, quantitative capabilities, simplicity, and cost-efficiency. 1H NMR is the method of choice for analysing a broad range of relatively abundant metabolites, which can be used to capture the plant chemical profile at one point in time and understand the pathways underpinning plant defence. We aim to highlight the challenges and advantages of using 1H NMR in plant metabolomics, interpret common trends observed in metabolite responses to biotic stress, suggest guidelines for method development and establish standard procedures. In our research, we explored the utility of 1H NMR in plant stress metabolomics, integrating bioassay results and plant resistance measurements to identify underlying key metabolic processes. Through the multivariate statistical analysis, we screened resistant and susceptible gerbera varieties, identifying polyketides as important contributors to resistance against powdery mildew and leading to a metabolome-based prediction model for mildew resistance for gerbera breeders. Reducing nitrate fertilisation levels in gerbera increased resistance to powdery mildew by enhancing polyketide and phenylpropanoid concentrations. Induction with methyl jasmonate of tomato plants grown in different substrates increased trip defence, stimulating an increase in phenylpropanoid pathway metabolites. Our results highlight the crucial role of secondary metabolites in plant defence. At the same time, our findings underscore the strengths and limitations of 1H NMR in studying plant defence pathways, highlighting when it outperforms liquid chromatography coupled with mass spectrometry (LC-MS) and when LC-MS is preferable. The proposed methodological guidelines and standardised procedures enhance reproducibility, cross-study comparability, and the broader adoption of 1H NMR in plant metabolomics.

Název v anglickém jazyce

Strength of 1H NMR in Plant Science and Biotic Stress Research

Popis výsledku anglicky

Metabolomics has become an important tool in elucidating the complex relationship between a plant genotype and phenotype. For over 20 years, nuclear magnetic resonance (NMR) spectroscopy has been known for its robustness, quantitative capabilities, simplicity, and cost-efficiency. 1H NMR is the method of choice for analysing a broad range of relatively abundant metabolites, which can be used to capture the plant chemical profile at one point in time and understand the pathways underpinning plant defence. We aim to highlight the challenges and advantages of using 1H NMR in plant metabolomics, interpret common trends observed in metabolite responses to biotic stress, suggest guidelines for method development and establish standard procedures. In our research, we explored the utility of 1H NMR in plant stress metabolomics, integrating bioassay results and plant resistance measurements to identify underlying key metabolic processes. Through the multivariate statistical analysis, we screened resistant and susceptible gerbera varieties, identifying polyketides as important contributors to resistance against powdery mildew and leading to a metabolome-based prediction model for mildew resistance for gerbera breeders. Reducing nitrate fertilisation levels in gerbera increased resistance to powdery mildew by enhancing polyketide and phenylpropanoid concentrations. Induction with methyl jasmonate of tomato plants grown in different substrates increased trip defence, stimulating an increase in phenylpropanoid pathway metabolites. Our results highlight the crucial role of secondary metabolites in plant defence. At the same time, our findings underscore the strengths and limitations of 1H NMR in studying plant defence pathways, highlighting when it outperforms liquid chromatography coupled with mass spectrometry (LC-MS) and when LC-MS is preferable. The proposed methodological guidelines and standardised procedures enhance reproducibility, cross-study comparability, and the broader adoption of 1H NMR in plant metabolomics.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40101 - Agriculture

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů