Mutual interaction of silymarin flavonolignans with human gut microbiota

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102222" target="_blank" >RIV/60460709:41210/25:102222 - isvavai.cz</a>

Výsledek na webu

<a href="https://amsacta.unibo.it/id/eprint/8570/3/Book%20of%20Proceedings%201st%20INFOGUT%20ANNUAL%20MEETING%20complete%2024%2010%202025.pdf" target="_blank" >https://amsacta.unibo.it/id/eprint/8570/3/Book%20of%20Proceedings%201st%20INFOGUT%20ANNUAL%20MEETING%20complete%2024%2010%202025.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mutual interaction of silymarin flavonolignans with human gut microbiota

Popis výsledku v původním jazyce

Introduction. Silymarin, an extract from fruits of the milk thistle Silybum marianum (L.) Gaertn., is made up of the flavonolignans silybin A and B, isosilybin A and B, silychristin A and B, silydianin and the flavanol taxifolin. Minor components include isosilychristin, 2,3- dehydrosilybin, 2,3-dehydrosilychristin and 2,3-dehydrosilydianin. The bioavailability of silymarin components is 20–50% due to low. The aim of the study was to evaluate the complex interactions of silymarin and its components with gut microbiota both ex vivo and in vivo. Materials and Methods. Biotransformation of silymarin components was studied ex vivo, using batch incubations inoculated by fecal slurry and HPLC/MS. The interaction of the silymarin complex was investigated with a focus on aging populations. Using advanced techniques such as NGS, NMR and LC-MS, we analyzed the dual impact of the microbiome on silymarin metabolism and the effect of silymarin on the microbiome’s structure and function. Finally, in 33 healthy adult male volunteers, who received 200 mg of silymarin orally twice daily for three months, formation of metabolites in urine and feces was evaluated by HPLC/MS and bacterial composition of feces was investigated by NGS. Results and Discussion. At 200 mg/L the flavonolignans were resistant to the metabolic action of microbiota. At 10 mg/L, biotransformation of flavonolignans was much slower than that of taxifolin. Silybin, isosilybin, and 2,3-dehydrosilybin underwent mostly demethylation, silychristin was predominantly reduced. Silydianin, 2,3-dehydrosilychristin and 2,3- dehydrosilydianin were reduced and decarbonylation and cysteine conjugation proceeded. No low-molecular-weight phenolic metabolites were detected. Silymarin significantly altered the metabolism of the gut microbiota, decreasing short-chain fatty acid production and glucose utilization. Healthy elders (70–80 years) showed a significant increase in a specific catabolite associated with Oscillibacter. Conversely, healthy young donors (12–45 years) exhibited faster breakdown of silymarin components, particularly isosilybin B, which negatively correlated with higher abundance of Faecalibacterium and Erysipelotrochaceae UCG-003. In volunteers, a correlation was found between the number of metabolites and the composition of the intestinal microbiota. Conclusion. Silymarin interacts with the microbiome, influences its composition and metabolic profile. The amount of metabolites in feces depend on the composition of the gut microbiota that is responsible for large inter-individual differences

Název v anglickém jazyce

Mutual interaction of silymarin flavonolignans with human gut microbiota

Popis výsledku anglicky

Introduction. Silymarin, an extract from fruits of the milk thistle Silybum marianum (L.) Gaertn., is made up of the flavonolignans silybin A and B, isosilybin A and B, silychristin A and B, silydianin and the flavanol taxifolin. Minor components include isosilychristin, 2,3- dehydrosilybin, 2,3-dehydrosilychristin and 2,3-dehydrosilydianin. The bioavailability of silymarin components is 20–50% due to low. The aim of the study was to evaluate the complex interactions of silymarin and its components with gut microbiota both ex vivo and in vivo. Materials and Methods. Biotransformation of silymarin components was studied ex vivo, using batch incubations inoculated by fecal slurry and HPLC/MS. The interaction of the silymarin complex was investigated with a focus on aging populations. Using advanced techniques such as NGS, NMR and LC-MS, we analyzed the dual impact of the microbiome on silymarin metabolism and the effect of silymarin on the microbiome’s structure and function. Finally, in 33 healthy adult male volunteers, who received 200 mg of silymarin orally twice daily for three months, formation of metabolites in urine and feces was evaluated by HPLC/MS and bacterial composition of feces was investigated by NGS. Results and Discussion. At 200 mg/L the flavonolignans were resistant to the metabolic action of microbiota. At 10 mg/L, biotransformation of flavonolignans was much slower than that of taxifolin. Silybin, isosilybin, and 2,3-dehydrosilybin underwent mostly demethylation, silychristin was predominantly reduced. Silydianin, 2,3-dehydrosilychristin and 2,3- dehydrosilydianin were reduced and decarbonylation and cysteine conjugation proceeded. No low-molecular-weight phenolic metabolites were detected. Silymarin significantly altered the metabolism of the gut microbiota, decreasing short-chain fatty acid production and glucose utilization. Healthy elders (70–80 years) showed a significant increase in a specific catabolite associated with Oscillibacter. Conversely, healthy young donors (12–45 years) exhibited faster breakdown of silymarin components, particularly isosilybin B, which negatively correlated with higher abundance of Faecalibacterium and Erysipelotrochaceae UCG-003. In volunteers, a correlation was found between the number of metabolites and the composition of the intestinal microbiota. Conclusion. Silymarin interacts with the microbiome, influences its composition and metabolic profile. The amount of metabolites in feces depend on the composition of the gut microbiota that is responsible for large inter-individual differences

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

30308 - Nutrition, Dietetics

Projekt

<a href="/cs/project/GA23-04655S" target="_blank" >GA23-04655S: Role prenylace a glykosylace v protizánětlivé aktivitě a metabolismu přírodních fenolových látek</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of Conference Proceedings 1 st INFOGUT Annual Meeting

ISBN

9788854972100

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

16-17

Název nakladatele

University of Bologna

Místo vydání

Bologna

Místo konání akce

Bologna

Datum konání akce

1. 1. 2025

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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