Drought Response and Genetic Variation in Scots Pine Seedlings' Provenances: Insights From High-Throughput Phenotyping for Climate-Resilient Forestry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102232" target="_blank" >RIV/60460709:41210/25:102232 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41320/25:102232

Výsledek na webu

<a href="https://doi.org/10.1111/eva.70157" target="_blank" >https://doi.org/10.1111/eva.70157</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/eva.70157" target="_blank" >10.1111/eva.70157</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Drought Response and Genetic Variation in Scots Pine Seedlings' Provenances: Insights From High-Throughput Phenotyping for Climate-Resilient Forestry

Popis výsledku v původním jazyce

Scots pine (Pinus sylvestris L.) is characterized by considerable intraspecific adaptive variability in response to environmental stress factors due to its wide geographical range. Adaptability is key for forestry, promising resilience against upcoming Europe's climate-driven droughts. We studied three provenances of pedigreed Scots pine seedlings from distinct upland and lowland habitats in the Czech Republic. A water deficit was induced in 2-year-old, potted seedlings in a greenhouse. Their physiological responses to drought were investigated at the beginning of growing season during the development of new shoots, and after subsequent summer rewatering. (1) We analyzed several physiological traits to assess their effectiveness in detecting treatment effects: steady-state quantum yield of PSII (QY Lss), maximum quantum yield of PSII (QY max), steady-state non-photochemical quenching (NPQ Lss), needle chlorophyll fluorescence ratio (SFR_R), and needle temperature normalized to ambient temperature (triangle T), using a high-throughput phenotyping unit. The divergence in SFR_R, QY max, QY Lss, NPQ Lss, and Delta T suggests that drought stress significantly impacts photosynthetic efficiency and heat dissipation, with recovery occurring after rewatering. (2) We detected differences within and among provenances utilizing a single nucleotide polymorphism genotyping array and linear mixed models integrating estimated genomic relationships to investigate genetic variation in needle functional traits in time. Throughout the experiment, heritability (h(2) ) varied widely among traits-with QY max and QY Lss showing the greatest variability (from 0 to 0.37), NPQ Lss exhibiting a narrower range aside from two outlier peaks, and SFR_R and triangle T displaying lower variability and lower h(2) values (0-0.24). The photosynthesis-related traits (QY max, QY Lss) showed the highest genetic variation, underscoring their potential for early-age phenotyping and selection of drought-tolerant genotypes. These findings address practical problems in forest management, particularly in light of changing weather patterns and climate variability, and provide a foundation for advanced optically based, early-age phenotyping to enhance forest resilience.

Název v anglickém jazyce

Drought Response and Genetic Variation in Scots Pine Seedlings' Provenances: Insights From High-Throughput Phenotyping for Climate-Resilient Forestry

Popis výsledku anglicky

Scots pine (Pinus sylvestris L.) is characterized by considerable intraspecific adaptive variability in response to environmental stress factors due to its wide geographical range. Adaptability is key for forestry, promising resilience against upcoming Europe's climate-driven droughts. We studied three provenances of pedigreed Scots pine seedlings from distinct upland and lowland habitats in the Czech Republic. A water deficit was induced in 2-year-old, potted seedlings in a greenhouse. Their physiological responses to drought were investigated at the beginning of growing season during the development of new shoots, and after subsequent summer rewatering. (1) We analyzed several physiological traits to assess their effectiveness in detecting treatment effects: steady-state quantum yield of PSII (QY Lss), maximum quantum yield of PSII (QY max), steady-state non-photochemical quenching (NPQ Lss), needle chlorophyll fluorescence ratio (SFR_R), and needle temperature normalized to ambient temperature (triangle T), using a high-throughput phenotyping unit. The divergence in SFR_R, QY max, QY Lss, NPQ Lss, and Delta T suggests that drought stress significantly impacts photosynthetic efficiency and heat dissipation, with recovery occurring after rewatering. (2) We detected differences within and among provenances utilizing a single nucleotide polymorphism genotyping array and linear mixed models integrating estimated genomic relationships to investigate genetic variation in needle functional traits in time. Throughout the experiment, heritability (h(2) ) varied widely among traits-with QY max and QY Lss showing the greatest variability (from 0 to 0.37), NPQ Lss exhibiting a narrower range aside from two outlier peaks, and SFR_R and triangle T displaying lower variability and lower h(2) values (0-0.24). The photosynthesis-related traits (QY max, QY Lss) showed the highest genetic variation, underscoring their potential for early-age phenotyping and selection of drought-tolerant genotypes. These findings address practical problems in forest management, particularly in light of changing weather patterns and climate variability, and provide a foundation for advanced optically based, early-age phenotyping to enhance forest resilience.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

<a href="/cs/project/LTAUSA19113" target="_blank" >LTAUSA19113: Využití genetické variability hyperspektrální odrazivosti ekotypů borovice lesní pro selekci jedinců odolných vůči suchu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Evolutionary Applications

ISSN

1752-4563

e-ISSN

1752-4571

Svazek periodika

18

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

21

Strana od-do

1-21

Kód UT WoS článku

001589285500001

EID výsledku v databázi Scopus

2-s2.0-105018501308