Molecular Identification of Zoonotic Taeniids Metacestodes in Several Rodent Species Trapped in Central Europe

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102898" target="_blank" >RIV/60460709:41210/25:102898 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1571082/full" target="_blank" >https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1571082/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fvets.2025.1571082" target="_blank" >10.3389/fvets.2025.1571082</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular Identification of Zoonotic Taeniids Metacestodes in Several Rodent Species Trapped in Central Europe

Popis výsledku v původním jazyce

Background Larval stages of carnivore cestodes in rodents have been extensively studied for decades, primarily based on morphological indicators. Numerous datasets, particularly from Central Europe, exist on this topic. Traditionally, the shape, number, and size of hooks have been key distinguishing features. However, recent findings suggest that morphology alone may not provide accurate identification. In this study, rodent livers from various habitats across five regions of the Czech Republic were examined for the presence of taeniid larval cestodes.Methods A total of 2,934 rodent specimens were collected using snap traps between 2014 and 2022. Taeniid metacestodes detected in these intermediate hosts were subsequently subjected to species determination through molecular (targeting the cytochrome oxidase subunit I gene) and morphological examinations.Results The following cestodes were identified using molecular methods: Hydatigera kamiyai (found in Apodemus flavicollis, Apodemus sylvaticus, Microtus arvalis, and Microtus agrestis), Taenia martis (in A. flavicollis, M. arvalis, and Myodes glareolus), and Versteria mustelae (in M. arvalis and My. glareolus). Sequencing of 55 PCR-positive isolates revealed 13 haplotypes of H. kamiyai, one of T. martis and four of V. mustelae. Further, we also identified co-infection by more metacestode species or haplotypes. No rodent liver samples tested were positive for Echinococcus multilocularis or Hydatigera taeniaeformis sensu stricto, either through morphological or molecular identification methods. Furthermore, no other cryptic species were detected during this study.Conclusion This study presents the first comprehensive molecular data on the aforementioned zoonotic cestode species in the Czech Republic. The findings demonstrate the importance of using molecular techniques for Taeniidae species determination, as morphological methods may lead to erroneous classifications. Furthermore, this research highlights the importance of accurate diagnostic techniques, enabling the development of effective prevention and control strategies by verifying host-parasite relationships.

Název v anglickém jazyce

Molecular Identification of Zoonotic Taeniids Metacestodes in Several Rodent Species Trapped in Central Europe

Popis výsledku anglicky

Background Larval stages of carnivore cestodes in rodents have been extensively studied for decades, primarily based on morphological indicators. Numerous datasets, particularly from Central Europe, exist on this topic. Traditionally, the shape, number, and size of hooks have been key distinguishing features. However, recent findings suggest that morphology alone may not provide accurate identification. In this study, rodent livers from various habitats across five regions of the Czech Republic were examined for the presence of taeniid larval cestodes.Methods A total of 2,934 rodent specimens were collected using snap traps between 2014 and 2022. Taeniid metacestodes detected in these intermediate hosts were subsequently subjected to species determination through molecular (targeting the cytochrome oxidase subunit I gene) and morphological examinations.Results The following cestodes were identified using molecular methods: Hydatigera kamiyai (found in Apodemus flavicollis, Apodemus sylvaticus, Microtus arvalis, and Microtus agrestis), Taenia martis (in A. flavicollis, M. arvalis, and Myodes glareolus), and Versteria mustelae (in M. arvalis and My. glareolus). Sequencing of 55 PCR-positive isolates revealed 13 haplotypes of H. kamiyai, one of T. martis and four of V. mustelae. Further, we also identified co-infection by more metacestode species or haplotypes. No rodent liver samples tested were positive for Echinococcus multilocularis or Hydatigera taeniaeformis sensu stricto, either through morphological or molecular identification methods. Furthermore, no other cryptic species were detected during this study.Conclusion This study presents the first comprehensive molecular data on the aforementioned zoonotic cestode species in the Czech Republic. The findings demonstrate the importance of using molecular techniques for Taeniidae species determination, as morphological methods may lead to erroneous classifications. Furthermore, this research highlights the importance of accurate diagnostic techniques, enabling the development of effective prevention and control strategies by verifying host-parasite relationships.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/GA23-07811S" target="_blank" >GA23-07811S: Interakce parazitů a kovů (metaloidů) v drobných zemních savcích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FRONTIERS IN VETERINARY SCIENCE

ISSN

2297-1769

e-ISSN

2297-1769

Svazek periodika

12

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

1-13

Kód UT WoS článku

001499962000001

EID výsledku v databázi Scopus

2-s2.0-105006976782