Exogenous Proline Modulates Physiological Responses and Induces Stress Memory in Wheat Under Repeated and Delayed Drought Stress

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102937" target="_blank" >RIV/60460709:41210/25:102937 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2073-4395/15/6/1370" target="_blank" >https://www.mdpi.com/2073-4395/15/6/1370</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy15061370" target="_blank" >10.3390/agronomy15061370</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Exogenous Proline Modulates Physiological Responses and Induces Stress Memory in Wheat Under Repeated and Delayed Drought Stress

Popis výsledku v původním jazyce

Drought stress negatively affects plant metabolism and growth, triggering complex defence mechanisms to limit damage. This study evaluated the effectiveness of a single foliar application of 1 mM L-proline (Pro) in winter wheat (Triticum aestivum L., cv. Bohemie) in two separate experiments differing in the time interval between application and drought-7 days (experiment 1) and 35 days (experiment 2). Net photosynthetic rate (A), transpiration rate (E), stomatal conductance (gs), leaf water potential (Psi w), intrinsic water use efficiency (WUEi), endogenous proline content (Pro), malondialdehyde content (MDA), and maximum quantum yield of photosystem II (F-v/F-m) were measured. In experiment 1, drought markedly reduced net photosynthetic rate, transpiration rate, stomatal conductance, and leaf water potential in both drought-stressed treatments, namely, without priming plants (S) and with Pro priming plants (SPro). Pro and MDA content increased under stress. Higher E and gs in the SPro treatment indicated more effective stomatal regulation and a distinct water use strategy. Pro content was significantly lower in SPro compared to S, whereas differences in MDA levels between these treatments were not statistically significant. The second drought period (D2) led to more pronounced limitations in gas exchange in both S and SPro. Enhanced osmoregulation was reflected by lower Psi w (S < SPro) and higher Pro accumulation in S (S > SPro). The effect of exogenous Pro persisted in the form of reduced endogenous Pro synthesis and improved photosystem II protection. Rehydration of stressed plants restored all monitored physiological parameters, and Pro-treated plants exhibited a more efficient recovery of gas exchange. Experiment 2 demonstrated a long-lasting priming effect that improved the preparedness of plants for future drought events. In the SPro treatment, this stress memory supported more efficient osmoregulation, reduced lipid peroxidation, improved protection of photosystem II integrity, and a more effective restart of gas exchange following rehydration. Our findings highlight the potential of exogenous proline as a practical tool for enhancing crop resilience to climate-induced drought stress.

Název v anglickém jazyce

Exogenous Proline Modulates Physiological Responses and Induces Stress Memory in Wheat Under Repeated and Delayed Drought Stress

Popis výsledku anglicky

Drought stress negatively affects plant metabolism and growth, triggering complex defence mechanisms to limit damage. This study evaluated the effectiveness of a single foliar application of 1 mM L-proline (Pro) in winter wheat (Triticum aestivum L., cv. Bohemie) in two separate experiments differing in the time interval between application and drought-7 days (experiment 1) and 35 days (experiment 2). Net photosynthetic rate (A), transpiration rate (E), stomatal conductance (gs), leaf water potential (Psi w), intrinsic water use efficiency (WUEi), endogenous proline content (Pro), malondialdehyde content (MDA), and maximum quantum yield of photosystem II (F-v/F-m) were measured. In experiment 1, drought markedly reduced net photosynthetic rate, transpiration rate, stomatal conductance, and leaf water potential in both drought-stressed treatments, namely, without priming plants (S) and with Pro priming plants (SPro). Pro and MDA content increased under stress. Higher E and gs in the SPro treatment indicated more effective stomatal regulation and a distinct water use strategy. Pro content was significantly lower in SPro compared to S, whereas differences in MDA levels between these treatments were not statistically significant. The second drought period (D2) led to more pronounced limitations in gas exchange in both S and SPro. Enhanced osmoregulation was reflected by lower Psi w (S < SPro) and higher Pro accumulation in S (S > SPro). The effect of exogenous Pro persisted in the form of reduced endogenous Pro synthesis and improved photosystem II protection. Rehydration of stressed plants restored all monitored physiological parameters, and Pro-treated plants exhibited a more efficient recovery of gas exchange. Experiment 2 demonstrated a long-lasting priming effect that improved the preparedness of plants for future drought events. In the SPro treatment, this stress memory supported more efficient osmoregulation, reduced lipid peroxidation, improved protection of photosystem II integrity, and a more effective restart of gas exchange following rehydration. Our findings highlight the potential of exogenous proline as a practical tool for enhancing crop resilience to climate-induced drought stress.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40101 - Agriculture

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agronomy

ISSN

2073-4395

e-ISSN

2073-4395

Svazek periodika

15

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

17

Strana od-do

1-17

Kód UT WoS článku

001515673900001

EID výsledku v databázi Scopus

2-s2.0-105008962094