New microplate disc volatilization assay for<i> in</i><i> vitro</i> evaluation of toxicity and antimicrobial activity of volatiles in vapour phase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A103012" target="_blank" >RIV/60460709:41210/25:103012 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41340/25:103012

Výsledek na webu

<a href="https://doi.org/10.1016/j.fct.2025.115744" target="_blank" >https://doi.org/10.1016/j.fct.2025.115744</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fct.2025.115744" target="_blank" >10.1016/j.fct.2025.115744</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New microplate disc volatilization assay for<i> in</i><i> vitro</i> evaluation of toxicity and antimicrobial activity of volatiles in vapour phase

Popis výsledku v původním jazyce

In this study, a novel microplate disc volatilization method for in vitro screening of cytotoxic and antibacterial potential of volatile agents in the vapour phase was designed with the aim to assess their therapeutic potential for development of new inhalation therapies. The toxicity and antibacterial activity of plant-derived volatiles (allyl isothiocyanate, 8-quinolinol, thymohydroquinone, thymoquinone) and essential oils (EOs) from Colebrookea oppositifolia leaf and Neolitsea cassia bark were evaluated against human lung fibroblasts MRC-5 and respiratory pathogens. Allyl isothiocyanate and C. oppositifolia EO did not affect the cell viability (IC50 > 160 mu g/cm(3)), on the other hand thymohydroquinone exhibited the highest cytotoxic effect (IC50 = 5.85 mu g/cm(3)) followed by thymoquinone and N. cassia EO (IC50 = 7.95 and 18.58 mu g/cm(3), respectively). 8-Quinolinol possessed the strongest antibacterial effect against Staphylococcus aureus and Heamophilus influenzae with respective minimum inhibitory concentrations of 0.625 and 1.25 mu g/cm(3). Calculated therapeutic index (>21.05) suggests relative inhalation safety of this compound. Using a gas chromatography/mass spectrometry analysis, E-cinnmaldehyde, benzaldehyde, and eucalyptol were identified as major components of N. cassia bark oil, while leaf oil of C. oppositifolia consisted predominantly of thunbergene, beta-caryophyllene, and m-camphorene. Moreover, chemical profile of N. cassia EO in the vapour phase showed E-cinnamaldehyde, o-cymene, and alpha-pinene as the most abundant volatiles. The results suggest 8-quinolinol for further research focused on development of inhalation therapies. Additionally, the results demonstrate validity of the microplate disc volatilization method, which allows cost and labour effective high-throughput screening of cytotoxicity and antimicrobial activity of volatiles in vapour phase.

Název v anglickém jazyce

New microplate disc volatilization assay for<i> in</i><i> vitro</i> evaluation of toxicity and antimicrobial activity of volatiles in vapour phase

Popis výsledku anglicky

In this study, a novel microplate disc volatilization method for in vitro screening of cytotoxic and antibacterial potential of volatile agents in the vapour phase was designed with the aim to assess their therapeutic potential for development of new inhalation therapies. The toxicity and antibacterial activity of plant-derived volatiles (allyl isothiocyanate, 8-quinolinol, thymohydroquinone, thymoquinone) and essential oils (EOs) from Colebrookea oppositifolia leaf and Neolitsea cassia bark were evaluated against human lung fibroblasts MRC-5 and respiratory pathogens. Allyl isothiocyanate and C. oppositifolia EO did not affect the cell viability (IC50 > 160 mu g/cm(3)), on the other hand thymohydroquinone exhibited the highest cytotoxic effect (IC50 = 5.85 mu g/cm(3)) followed by thymoquinone and N. cassia EO (IC50 = 7.95 and 18.58 mu g/cm(3), respectively). 8-Quinolinol possessed the strongest antibacterial effect against Staphylococcus aureus and Heamophilus influenzae with respective minimum inhibitory concentrations of 0.625 and 1.25 mu g/cm(3). Calculated therapeutic index (>21.05) suggests relative inhalation safety of this compound. Using a gas chromatography/mass spectrometry analysis, E-cinnmaldehyde, benzaldehyde, and eucalyptol were identified as major components of N. cassia bark oil, while leaf oil of C. oppositifolia consisted predominantly of thunbergene, beta-caryophyllene, and m-camphorene. Moreover, chemical profile of N. cassia EO in the vapour phase showed E-cinnamaldehyde, o-cymene, and alpha-pinene as the most abundant volatiles. The results suggest 8-quinolinol for further research focused on development of inhalation therapies. Additionally, the results demonstrate validity of the microplate disc volatilization method, which allows cost and labour effective high-throughput screening of cytotoxicity and antimicrobial activity of volatiles in vapour phase.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FOOD AND CHEMICAL TOXICOLOGY

ISSN

0278-6915

e-ISSN

1873-6351

Svazek periodika

206

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

14

Strana od-do

—

Kód UT WoS článku

001583006000001

EID výsledku v databázi Scopus

2-s2.0-105017892525