Effects of Defined Pig Microbiota on Acute Salmonellosis in Gnotobiotic Piglets

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A106438" target="_blank" >RIV/60460709:41210/25:106438 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/25:10515647

Výsledek na webu

<a href="https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202503234R" target="_blank" >https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202503234R</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1096/fj.202503234" target="_blank" >10.1096/fj.202503234</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of Defined Pig Microbiota on Acute Salmonellosis in Gnotobiotic Piglets

Popis výsledku v původním jazyce

Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is a ubiquitous Salmonella serovar that causes enterocolitis in humans, livestock, poultry, and some pets. Gnotobiotic animals are suitable for studying the development of initial colonization in the gastrointestinal tract, host-microbiota cross-talk, and interactions among microbiota strains. To clarify the impact of pre-colonization of the germ-free piglets with defined pig microbiota (DPM) on the subsequent infection with S. Typhimurium LT2 (LT2), we focused on the acute host immune response. Colonization with DPM did not cause signs of enterocolitis (sleepiness, anorexia, fever, and diarrhea), histological changes in the intestine, the density of acid mucin-producing cells, expression of villin, claudin-1, occludin, TLR4, MD-2, CD14, LBP, MyD88, and TRIF, induction of inflammatory cytokines IL-1ß, IL-6, IL-8, IL-10, IL-12/23p40, TNF-?, IFN-?, and HMGB1. LT2 translocated into the mesenteric lymph nodes, liver, spleen, and blood of the LT2 group, as well as damaged the intestinal structure, and upregulated intestinal inflammatory cytokine levels. The pre-colonization with DPM ameliorated the LT2-induced signs of enterocolitis, intestinal damage, and inflammatory changes. Changes in intestinal levels of soluble TLR4 and TLR2 indicate the possible inclusion of soluble TLRs in the regulation of the inflammatory process. The controlled initial colonization of the newborn gastrointestinal tract with a defined microbial consortium can increase newborn resistance to enteric pathogens and promote thriving of young animals.

Název v anglickém jazyce

Effects of Defined Pig Microbiota on Acute Salmonellosis in Gnotobiotic Piglets

Popis výsledku anglicky

Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is a ubiquitous Salmonella serovar that causes enterocolitis in humans, livestock, poultry, and some pets. Gnotobiotic animals are suitable for studying the development of initial colonization in the gastrointestinal tract, host-microbiota cross-talk, and interactions among microbiota strains. To clarify the impact of pre-colonization of the germ-free piglets with defined pig microbiota (DPM) on the subsequent infection with S. Typhimurium LT2 (LT2), we focused on the acute host immune response. Colonization with DPM did not cause signs of enterocolitis (sleepiness, anorexia, fever, and diarrhea), histological changes in the intestine, the density of acid mucin-producing cells, expression of villin, claudin-1, occludin, TLR4, MD-2, CD14, LBP, MyD88, and TRIF, induction of inflammatory cytokines IL-1ß, IL-6, IL-8, IL-10, IL-12/23p40, TNF-?, IFN-?, and HMGB1. LT2 translocated into the mesenteric lymph nodes, liver, spleen, and blood of the LT2 group, as well as damaged the intestinal structure, and upregulated intestinal inflammatory cytokine levels. The pre-colonization with DPM ameliorated the LT2-induced signs of enterocolitis, intestinal damage, and inflammatory changes. Changes in intestinal levels of soluble TLR4 and TLR2 indicate the possible inclusion of soluble TLRs in the regulation of the inflammatory process. The controlled initial colonization of the newborn gastrointestinal tract with a defined microbial consortium can increase newborn resistance to enteric pathogens and promote thriving of young animals.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

<a href="/cs/project/GA21-15621S" target="_blank" >GA21-15621S: Definovaná minimální mikrobiota v ochraně proti potravinovému patogenu Salmonella enterica</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FASEB JOURNAL

ISSN

0892-6638

e-ISSN

0892-6638

Svazek periodika

40

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

17

Strana od-do

1-17

Kód UT WoS článku

001651972700001

EID výsledku v databázi Scopus

2-s2.0-105026349833