The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAir(TM)) and in Human Bronchial Epithelial Cells (BEAS-2B)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41310%2F19%3A81925" target="_blank" >RIV/60460709:41310/19:81925 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68378041:_____/19:00518531 RIV/68407700:21220/19:00335128 RIV/68407700:21230/19:00335128 RIV/00216208:11310/19:10409254
Výsledek na webu
<a href="https://www.mdpi.com/1422-0067/20/22/5710" target="_blank" >https://www.mdpi.com/1422-0067/20/22/5710</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms20225710" target="_blank" >10.3390/ijms20225710</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAir(TM)) and in Human Bronchial Epithelial Cells (BEAS-2B)
Popis výsledku v původním jazyce
The biological effects induced by complete engine emissions in a 3D model of the human airway (MucilAir(TM)) and in human bronchial epithelial cells (BEAS-2B) grown at the air-liquid interface were compared. The cells were exposed for one or five days to emissions generated by a Euro 5 direct injection spark ignition engine. The general condition of the cells was assessed by the measurement of transepithelial electrical resistance and mucin production. The cytotoxic effects were evaluated by adenylate kinase (AK) and lactate dehydrogenase (LDH) activity. Phosphorylation of histone H2AX was used to detect double-stranded DNA breaks. The expression of the selected 370 relevant genes was analyzed using next-generation sequencing. The exposure had minimal effects on integrity and AK leakage in both cell models. LDH activity and mucin production in BEAS-2B cells significantly increased after longer exposures DNA breaks were also detected. The exposure affected CYP1A1 and HSPA5 expression in MucilAir(TM).
Název v anglickém jazyce
The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAir(TM)) and in Human Bronchial Epithelial Cells (BEAS-2B)
Popis výsledku anglicky
The biological effects induced by complete engine emissions in a 3D model of the human airway (MucilAir(TM)) and in human bronchial epithelial cells (BEAS-2B) grown at the air-liquid interface were compared. The cells were exposed for one or five days to emissions generated by a Euro 5 direct injection spark ignition engine. The general condition of the cells was assessed by the measurement of transepithelial electrical resistance and mucin production. The cytotoxic effects were evaluated by adenylate kinase (AK) and lactate dehydrogenase (LDH) activity. Phosphorylation of histone H2AX was used to detect double-stranded DNA breaks. The expression of the selected 370 relevant genes was analyzed using next-generation sequencing. The exposure had minimal effects on integrity and AK leakage in both cell models. LDH activity and mucin production in BEAS-2B cells significantly increased after longer exposures DNA breaks were also detected. The exposure affected CYP1A1 and HSPA5 expression in MucilAir(TM).
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Svazek periodika
2019
Číslo periodika v rámci svazku
20
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
22
Strana od-do
1-22
Kód UT WoS článku
000502786800192
EID výsledku v databázi Scopus
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