Age matters: Life-stage, tissue, and sex-specific gene expression dynamics in Ips typographus (Coleoptera: Curculionidae: Scolytinae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F23%3A97045" target="_blank" >RIV/60460709:41320/23:97045 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3389/ffgc.2023.1124754" target="_blank" >http://dx.doi.org/10.3389/ffgc.2023.1124754</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/ffgc.2023.1124754" target="_blank" >10.3389/ffgc.2023.1124754</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Age matters: Life-stage, tissue, and sex-specific gene expression dynamics in Ips typographus (Coleoptera: Curculionidae: Scolytinae)

Popis výsledku v původním jazyce

The Eurasian spruce bark beetle (ESBB), Ips typographus, has recently caused catastrophic damage to Norway spruce (Picea abies) forests in Europe, resulting in the loss of more than 100 million cubic meters of wood. Traditional forest management strategies have failed to constrain the growing infestation rate; hence, novel measures must be deployed. A better understanding of ESBB physiology and adaptation to host allelochemicals may provide a platform for future management strategies using molecular tools such as RNA interference. To understand ESBB physiology and adaptation, the current study unraveled the gene expression dynamics of ESBB in different life stages and tissues. We obtained ESBB transcriptomes for different life stages [larvae (L1, L2, and L3), pupa, callow, and sclerotized adult] and male/female tissues (gut, fat body, and head) from callow and sclerotized adult beetles. Differential gene expression analysis (DGE) identified multiple gene families related to detoxification, digestion, resistance, and transport in different life stages and tissues of the beetle. Gene Ontology (GO) enrichment revealed 61 critical metabolic pathways enriched across all DGE comparisons. DGE analysis further pinpointed the differential expression of essential genes involved in detoxification, digestion, transport, and defense in various tissues and life stages. RT-qPCR experiments and enzymatic assays corroborated the findings further. The catalogue of differentially expressed genes identified in ESBB could aid better understanding of ESBB physiology and adaptation to hosts and serve as targets for future RNAi-based ESBB management.

Název v anglickém jazyce

Age matters: Life-stage, tissue, and sex-specific gene expression dynamics in Ips typographus (Coleoptera: Curculionidae: Scolytinae)

Popis výsledku anglicky

The Eurasian spruce bark beetle (ESBB), Ips typographus, has recently caused catastrophic damage to Norway spruce (Picea abies) forests in Europe, resulting in the loss of more than 100 million cubic meters of wood. Traditional forest management strategies have failed to constrain the growing infestation rate; hence, novel measures must be deployed. A better understanding of ESBB physiology and adaptation to host allelochemicals may provide a platform for future management strategies using molecular tools such as RNA interference. To understand ESBB physiology and adaptation, the current study unraveled the gene expression dynamics of ESBB in different life stages and tissues. We obtained ESBB transcriptomes for different life stages [larvae (L1, L2, and L3), pupa, callow, and sclerotized adult] and male/female tissues (gut, fat body, and head) from callow and sclerotized adult beetles. Differential gene expression analysis (DGE) identified multiple gene families related to detoxification, digestion, resistance, and transport in different life stages and tissues of the beetle. Gene Ontology (GO) enrichment revealed 61 critical metabolic pathways enriched across all DGE comparisons. DGE analysis further pinpointed the differential expression of essential genes involved in detoxification, digestion, transport, and defense in various tissues and life stages. RT-qPCR experiments and enzymatic assays corroborated the findings further. The catalogue of differentially expressed genes identified in ESBB could aid better understanding of ESBB physiology and adaptation to hosts and serve as targets for future RNAi-based ESBB management.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FRONTIERS IN FORESTS AND GLOBAL CHANGE

ISSN

2624-893X

e-ISSN

2624-893X

Svazek periodika

6

Číslo periodika v rámci svazku

2023

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

26

Strana od-do

1-26

Kód UT WoS článku

000956681300001

EID výsledku v databázi Scopus

2-s2.0-85150962533