Comparative analysis of olfactory sensory neurons in two Ips species reveals conserved and species-specific olfactory adaptations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F25%3A103225" target="_blank" >RIV/60460709:41320/25:103225 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.frontiersin.org/journals/forests-and-global-change/articles/10.3389/ffgc.2025.1588866/full" target="_blank" >https://www.frontiersin.org/journals/forests-and-global-change/articles/10.3389/ffgc.2025.1588866/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/ffgc.2025.1588866" target="_blank" >10.3389/ffgc.2025.1588866</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative analysis of olfactory sensory neurons in two Ips species reveals conserved and species-specific olfactory adaptations

Popis výsledku v původním jazyce

Introduction Bark beetles spend most of their lives under the bark of trees, with some species being economically significant pests that cause widespread tree mortality. Their behavior is primarily driven by olfactory signals, with aggregation pheromones playing a prominent role alongside volatiles from hosts, non-host trees, and associated microbes. These signals are detected by olfactory sensory neurons (OSNs) housed in hair-like sensilla on the antennae. In this study, we focused on two Ips species with distinct host preferences: Ips acuminatus, which infests pine species, and Ips cembrae, which primarily attacks European larch. To better understand species-specific adaptations and shared olfactory mechanisms, we compared their olfactory responses with those of Ips typographus, a major pest of Norway spruce. By investigating the frequency, specificity, and antennal distribution of various OSN classes, we aimed to uncover both conserved and different olfactory mechanisms across Ips species with different host associations.Methods We conducted single sensillum recordings (SSR) to examine OSN responses in the antennal olfactory sensilla of I. acuminatus and I. cembrae. The responses were compared to existing data from I. typographus to identify potential species-specific adaptations and conserved olfactory mechanisms. A panel of 57 ecologically relevant odorants was tested, comprising interspecific and intraspecific pheromones, along with compounds associated with host- and non-host trees, as well as symbiotic fungi.Results and discussion Based on their response profiles, we identified nineteen OSN classes in both I. acuminatus and I. cembrae. A few selected OSN classes were further analyzed using dose-response tests to assess their specificity and sensitivity. Three OSN classes in I. acuminatus and four in I. cembrae were specific to host-related compounds. Two OSN classes responded to non-host volatiles, while one OSN class exhibited strong responses to microbial volatiles in both species. Several OSN classes specific to pheromone compounds, non-host, and microbial volatiles showed similar response profiles in both I. acuminatus and I. cembrae as well as in OSN classes previously reported in I. typographus, potentially reflecting close phylogenetic relationships and shared ecological traits among these species.

Název v anglickém jazyce

Comparative analysis of olfactory sensory neurons in two Ips species reveals conserved and species-specific olfactory adaptations

Popis výsledku anglicky

Introduction Bark beetles spend most of their lives under the bark of trees, with some species being economically significant pests that cause widespread tree mortality. Their behavior is primarily driven by olfactory signals, with aggregation pheromones playing a prominent role alongside volatiles from hosts, non-host trees, and associated microbes. These signals are detected by olfactory sensory neurons (OSNs) housed in hair-like sensilla on the antennae. In this study, we focused on two Ips species with distinct host preferences: Ips acuminatus, which infests pine species, and Ips cembrae, which primarily attacks European larch. To better understand species-specific adaptations and shared olfactory mechanisms, we compared their olfactory responses with those of Ips typographus, a major pest of Norway spruce. By investigating the frequency, specificity, and antennal distribution of various OSN classes, we aimed to uncover both conserved and different olfactory mechanisms across Ips species with different host associations.Methods We conducted single sensillum recordings (SSR) to examine OSN responses in the antennal olfactory sensilla of I. acuminatus and I. cembrae. The responses were compared to existing data from I. typographus to identify potential species-specific adaptations and conserved olfactory mechanisms. A panel of 57 ecologically relevant odorants was tested, comprising interspecific and intraspecific pheromones, along with compounds associated with host- and non-host trees, as well as symbiotic fungi.Results and discussion Based on their response profiles, we identified nineteen OSN classes in both I. acuminatus and I. cembrae. A few selected OSN classes were further analyzed using dose-response tests to assess their specificity and sensitivity. Three OSN classes in I. acuminatus and four in I. cembrae were specific to host-related compounds. Two OSN classes responded to non-host volatiles, while one OSN class exhibited strong responses to microbial volatiles in both species. Several OSN classes specific to pheromone compounds, non-host, and microbial volatiles showed similar response profiles in both I. acuminatus and I. cembrae as well as in OSN classes previously reported in I. typographus, potentially reflecting close phylogenetic relationships and shared ecological traits among these species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

<a href="/cs/project/GA23-07916S" target="_blank" >GA23-07916S: Identifikace a RNAi zhášení genů produkce agregačního feromonu agresivního kůrovce lýkožrouta smrkového (Ips typographus)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FRONTIERS IN FORESTS AND GLOBAL CHANGE

ISSN

2624-893X

e-ISSN

2624-893X

Svazek periodika

8

Číslo periodika v rámci svazku

2025

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

19

Strana od-do

1-19

Kód UT WoS článku

001510913200001

EID výsledku v databázi Scopus

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