RNAi biopesticides for precision control of invasive pests and pathogens in 3D forests: Quantum leap towards eco-friendly alternatives

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F25%3A103555" target="_blank" >RIV/60460709:41320/25:103555 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2352186425003906?pes=vor&utm_source=clarivate&getft_integrator=clarivate" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2352186425003906?pes=vor&utm_source=clarivate&getft_integrator=clarivate</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.eti.2025.104404" target="_blank" >10.1016/j.eti.2025.104404</a>

Jazyk výsledku

angličtina

Název v původním jazyce

RNAi biopesticides for precision control of invasive pests and pathogens in 3D forests: Quantum leap towards eco-friendly alternatives

Popis výsledku v původním jazyce

Climate change has severely amplified the frequency and severity of forest pest and pathogen outbreaks, leading to a decline in forest health, productivity, and biodiversity. Conventional pest management strategies, including chemical pesticides and mechanical removal, have proven largely ineffective or environmentally detrimental. RNA interference (RNAi) technology, particularly with nanotechnology, offers a promising and eco-friendly alternative for precisely controlling invasive forest pests and pathogens. Current review pinpoints significant advances in species-specific dsRNA design and delivery systems, including nanoparticle-based formulations such as chitosan, carbon quantum dots, liposomes, peptides, and layered double hydroxides that enhance the environmental stability, cellular uptake, and RNAi efficiency against different pests and pathogens. Moreover, delivery methods such as spray-induced and host-induced gene silencing (SIGS and HIGS) are also discussed, emphasising their feasibility in complex 3D forest environments. Recent advancements in software tools for species-specific dsRNA design, which democratise RNAi technology, were also presented. Finally, we assess the short-term and longterm environmental and ecotoxicological risks of RNAi-nanocomplexes, using foliar sprays and trunk injections, while highlighting the necessity to address species-specific sensitivity and ecological challenges, which are crucial for the market implementation of RNAi-nanobiopesticides. These assessments aim to increase forest resilience against biotic stressors under climate change and contribute to formulating a more refined, harmonised global framework for risk assessment of dsRNA-based biopesticides during large-scale forest applications.

Název v anglickém jazyce

RNAi biopesticides for precision control of invasive pests and pathogens in 3D forests: Quantum leap towards eco-friendly alternatives

Popis výsledku anglicky

Climate change has severely amplified the frequency and severity of forest pest and pathogen outbreaks, leading to a decline in forest health, productivity, and biodiversity. Conventional pest management strategies, including chemical pesticides and mechanical removal, have proven largely ineffective or environmentally detrimental. RNA interference (RNAi) technology, particularly with nanotechnology, offers a promising and eco-friendly alternative for precisely controlling invasive forest pests and pathogens. Current review pinpoints significant advances in species-specific dsRNA design and delivery systems, including nanoparticle-based formulations such as chitosan, carbon quantum dots, liposomes, peptides, and layered double hydroxides that enhance the environmental stability, cellular uptake, and RNAi efficiency against different pests and pathogens. Moreover, delivery methods such as spray-induced and host-induced gene silencing (SIGS and HIGS) are also discussed, emphasising their feasibility in complex 3D forest environments. Recent advancements in software tools for species-specific dsRNA design, which democratise RNAi technology, were also presented. Finally, we assess the short-term and longterm environmental and ecotoxicological risks of RNAi-nanocomplexes, using foliar sprays and trunk injections, while highlighting the necessity to address species-specific sensitivity and ecological challenges, which are crucial for the market implementation of RNAi-nanobiopesticides. These assessments aim to increase forest resilience against biotic stressors under climate change and contribute to formulating a more refined, harmonised global framework for risk assessment of dsRNA-based biopesticides during large-scale forest applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

20801 - Environmental biotechnology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ENVIRONMENTAL TECHNOLOGY & INNOVATION

ISSN

2352-1864

e-ISSN

2352-1864

Svazek periodika

40

Číslo periodika v rámci svazku

2025

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

34

Strana od-do

1-34

Kód UT WoS článku

001545718200004

EID výsledku v databázi Scopus

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