Aquatic invertebrate diversity profiling in heterogeneous wetland habitats by environmental DNA metabarcoding

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41330%2F23%3A94832" target="_blank" >RIV/60460709:41330/23:94832 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1470160X23002686" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1470160X23002686</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ecolind.2023.110126" target="_blank" >10.1016/j.ecolind.2023.110126</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Aquatic invertebrate diversity profiling in heterogeneous wetland habitats by environmental DNA metabarcoding

Popis výsledku v původním jazyce

Invertebrates play vital roles in maintaining biodiversity and food web structure. However, it is difficult to identify invertebrate taxa across complex habitats due to the limitation of traditional morphology. In subtropical wetlands, environmental DNA (eDNA) metabarcoding was used to characterize the composition and diversity of aquatic invertebrates and analyze the environmental impacts on invertebrate community structure. According to the relative abundance (%) of invertebrate OTU richness, 30 sampling sites in wetlands were clustered into six zones, which exhibited significant spatial (i.e., wetland type-specific) differences in taxonomic composition. The relative OTU abundance (%) at the phylum level showed that Cnidaria (48.5%) > Porifera (19.4%) > Rotifera (11.0%) > Mollusca (9.2%) > Annelida (5.3 +/- 2.8%) > others (less than 2.2%). Of the five alpha diversity in-dexes, 'Simpson' was the most effective index to distinguish the spatial differences in invertebrate diversity, and the class-level alpha diversity showed higher recognition than other taxonomic levels. The class-level biomarkers that could indicate the habitat-specific composition of local invertebrates were Insecta, Cnidaria, Hydrozoa, and Gastropoda in the inflow river; Ascidiacea and Demospongiae in the fluvial wetland; Clitellata, Gymnolaemata, and Monogononta in the lacustrine wetland; and Echinoidea in the estuarine wetland. The associations between environmental factors and invertebrate OTU richness based on redundancy analysis showed the taxon-level tendency of phylum (78.5%) > class (73.4%) > order (69.3%) > family (64.9%) > genus (61.2%). Our re-sults demonstrated that aquatic invertebrate diversity profiling by environmental DNA metabarcoding can effectively reflect the composition, diversity and biomarkers of invertebrate communities. In the future, because of its great application potential, the eDNA technique may play an important role in biomonitoring complex water environments.

Název v anglickém jazyce

Aquatic invertebrate diversity profiling in heterogeneous wetland habitats by environmental DNA metabarcoding

Popis výsledku anglicky

Invertebrates play vital roles in maintaining biodiversity and food web structure. However, it is difficult to identify invertebrate taxa across complex habitats due to the limitation of traditional morphology. In subtropical wetlands, environmental DNA (eDNA) metabarcoding was used to characterize the composition and diversity of aquatic invertebrates and analyze the environmental impacts on invertebrate community structure. According to the relative abundance (%) of invertebrate OTU richness, 30 sampling sites in wetlands were clustered into six zones, which exhibited significant spatial (i.e., wetland type-specific) differences in taxonomic composition. The relative OTU abundance (%) at the phylum level showed that Cnidaria (48.5%) > Porifera (19.4%) > Rotifera (11.0%) > Mollusca (9.2%) > Annelida (5.3 +/- 2.8%) > others (less than 2.2%). Of the five alpha diversity in-dexes, 'Simpson' was the most effective index to distinguish the spatial differences in invertebrate diversity, and the class-level alpha diversity showed higher recognition than other taxonomic levels. The class-level biomarkers that could indicate the habitat-specific composition of local invertebrates were Insecta, Cnidaria, Hydrozoa, and Gastropoda in the inflow river; Ascidiacea and Demospongiae in the fluvial wetland; Clitellata, Gymnolaemata, and Monogononta in the lacustrine wetland; and Echinoidea in the estuarine wetland. The associations between environmental factors and invertebrate OTU richness based on redundancy analysis showed the taxon-level tendency of phylum (78.5%) > class (73.4%) > order (69.3%) > family (64.9%) > genus (61.2%). Our re-sults demonstrated that aquatic invertebrate diversity profiling by environmental DNA metabarcoding can effectively reflect the composition, diversity and biomarkers of invertebrate communities. In the future, because of its great application potential, the eDNA technique may play an important role in biomonitoring complex water environments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ECOLOGICAL INDICATORS

ISSN

1470-160X

e-ISSN

1470-160X

Svazek periodika

150

Číslo periodika v rámci svazku

110126

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

12

Strana od-do

1-12

Kód UT WoS článku

000969878900001

EID výsledku v databázi Scopus

2-s2.0-85151485905