Triiron Tetraoxide (Fe3O4) Triggers Aerobic Denitrification Performance in Low C/N Water: Novel Insights into the Electron Transport System and Bacterial Community Model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41330%2F25%3A102073" target="_blank" >RIV/60460709:41330/25:102073 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acsestwater.5c00217" target="_blank" >https://pubs.acs.org/doi/10.1021/acsestwater.5c00217</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsestwater.5c00217" target="_blank" >10.1021/acsestwater.5c00217</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Triiron Tetraoxide (Fe3O4) Triggers Aerobic Denitrification Performance in Low C/N Water: Novel Insights into the Electron Transport System and Bacterial Community Model

Popis výsledku v původním jazyce

Denitrification performance is significantly influenced by electron donors, mainly organic electron donors. Inorganic electron donors (e.g., Fe3O4) have shown potential for enhancing the nitrate removal efficiency. However, the mechanism of Fe3O4 on denitrification under aerobic conditions remains poorly understood. This study investigated the synergistic mechanisms of electron transfer and microbial interactions facilitated by Fe3O4 in an aerobic denitrifying bacterial community isolated from a micropolluted reservoir. The results revealed that the aerobic denitrifying bacterial (ADB) reactor achieved over 50% efficiency in both denitrification and organic carbon removal. When Fe3O4 was added, the removal efficiencies for nitrate (NO3--N) and organic carbon increased to 82.38 and 66.84%, respectively. The adenosine triphosphate (ATP) activity and electron transport system activity (ETSA) increased by 1.3- and 1.7-fold, respectively, with the upregulation of denitrification-related enzymes. High-throughput sequencing analysis demonstrated that the bacterial community structure in the water column, biofilm, and sediment was enriched with Proteobacteria and Actinobacteria phyla in the biofilm. Additionally, statistical modeling further highlighted the stabilizing effects of Fe3O4 on the reaction system and the microbial community’s adaptive responses to environmental factors. In summary, this study provides a novel approach for leveraging Fe3O4 and aerobic denitrifying bacterial communities to achieve efficient nitrogen removal in micropolluted waters.

Název v anglickém jazyce

Triiron Tetraoxide (Fe3O4) Triggers Aerobic Denitrification Performance in Low C/N Water: Novel Insights into the Electron Transport System and Bacterial Community Model

Popis výsledku anglicky

Denitrification performance is significantly influenced by electron donors, mainly organic electron donors. Inorganic electron donors (e.g., Fe3O4) have shown potential for enhancing the nitrate removal efficiency. However, the mechanism of Fe3O4 on denitrification under aerobic conditions remains poorly understood. This study investigated the synergistic mechanisms of electron transfer and microbial interactions facilitated by Fe3O4 in an aerobic denitrifying bacterial community isolated from a micropolluted reservoir. The results revealed that the aerobic denitrifying bacterial (ADB) reactor achieved over 50% efficiency in both denitrification and organic carbon removal. When Fe3O4 was added, the removal efficiencies for nitrate (NO3--N) and organic carbon increased to 82.38 and 66.84%, respectively. The adenosine triphosphate (ATP) activity and electron transport system activity (ETSA) increased by 1.3- and 1.7-fold, respectively, with the upregulation of denitrification-related enzymes. High-throughput sequencing analysis demonstrated that the bacterial community structure in the water column, biofilm, and sediment was enriched with Proteobacteria and Actinobacteria phyla in the biofilm. Additionally, statistical modeling further highlighted the stabilizing effects of Fe3O4 on the reaction system and the microbial community’s adaptive responses to environmental factors. In summary, this study provides a novel approach for leveraging Fe3O4 and aerobic denitrifying bacterial communities to achieve efficient nitrogen removal in micropolluted waters.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10503 - Water resources

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS ES&T WATER

ISSN

2690-0637

e-ISSN

2690-0637

Svazek periodika

5

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

3391-3402

Kód UT WoS článku

001498441600001

EID výsledku v databázi Scopus

2-s2.0-105007071370