Eggs-posed: revision of <i>Schistosoma mansoni</i> venom allergen-like proteins unveils new genes and offers new insights into egg-host interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41330%2F25%3A106329" target="_blank" >RIV/60460709:41330/25:106329 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1186/s12864-025-11369-4" target="_blank" >https://doi.org/10.1186/s12864-025-11369-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-025-11369-4" target="_blank" >10.1186/s12864-025-11369-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Eggs-posed: revision of <i>Schistosoma mansoni</i> venom allergen-like proteins unveils new genes and offers new insights into egg-host interactions

Popis výsledku v původním jazyce

BackgroundVenom allergen-like proteins (VALs) are abundant in the excretory-secretory products (ESPs) of numerous parasitic helminths and have been extensively studied for over 30 years because of their potential to interact with host systems. Despite substantial research, however, the precise functions of these proteins remain largely unresolved. Schistosomes, parasites of the circulatory system, are no exception, with 29 SmVAL genes identified in the genome of Schistosoma mansoni to date. The eggs of these parasites, as primary pathogenic agents, interact directly with host tissues and release excretory-secretory products that aid their egress from the host. Although SmVALs have been detected in the egg secretome in the past, direct evidence of their secretion and functional interaction with host molecules has never been demonstrated. These findings fuel the ongoing debate as to whether egg-expressed SmVALs interact with the mammalian host or are rather miracidial proteins synthesized within the egg during larval development.ResultsBased on complete revision of the SmVAL family and an associated robust transcriptomic meta-analysis of gene expression across the life cycle, we show that many of SmVAL genes, including 6 newly identified genes, are expressed in the infective larvae-producing stages (eggs and sporocysts). Following localization of two "egg-specific" SmVAL9 and SmVAL29 did not prove active secretion of these molecules into surrounding tissues but were aligned with miracidial structures interfacing with the molluscan host, specifically the larval surface and penetration glands. Finally, we show the complete lack of interactions between candidate SmVAL proteins and an array of 755 human cell receptors via a state-of-the-art SAVEXIS screen.ConclusionsOverall, we conclude that these "egg" SmVALs are not involved in direct host-parasite interactions in the mammalian host and are rather proteins employed during intermediate host invasion. Our study revisits and updates the SmVAL gene family, highlighting the limitations of in silico protein function predictions while emphasizing the need for up-to-date datasets and tools together with experimental validation in host-parasite interactions. By uncovering the diversity, expression patterns, and interaction dynamics of SmVALs, we open new avenues for understanding host manipulation and reevaluating orthologous proteins in other helminths.

Název v anglickém jazyce

Eggs-posed: revision of <i>Schistosoma mansoni</i> venom allergen-like proteins unveils new genes and offers new insights into egg-host interactions

Popis výsledku anglicky

BackgroundVenom allergen-like proteins (VALs) are abundant in the excretory-secretory products (ESPs) of numerous parasitic helminths and have been extensively studied for over 30 years because of their potential to interact with host systems. Despite substantial research, however, the precise functions of these proteins remain largely unresolved. Schistosomes, parasites of the circulatory system, are no exception, with 29 SmVAL genes identified in the genome of Schistosoma mansoni to date. The eggs of these parasites, as primary pathogenic agents, interact directly with host tissues and release excretory-secretory products that aid their egress from the host. Although SmVALs have been detected in the egg secretome in the past, direct evidence of their secretion and functional interaction with host molecules has never been demonstrated. These findings fuel the ongoing debate as to whether egg-expressed SmVALs interact with the mammalian host or are rather miracidial proteins synthesized within the egg during larval development.ResultsBased on complete revision of the SmVAL family and an associated robust transcriptomic meta-analysis of gene expression across the life cycle, we show that many of SmVAL genes, including 6 newly identified genes, are expressed in the infective larvae-producing stages (eggs and sporocysts). Following localization of two "egg-specific" SmVAL9 and SmVAL29 did not prove active secretion of these molecules into surrounding tissues but were aligned with miracidial structures interfacing with the molluscan host, specifically the larval surface and penetration glands. Finally, we show the complete lack of interactions between candidate SmVAL proteins and an array of 755 human cell receptors via a state-of-the-art SAVEXIS screen.ConclusionsOverall, we conclude that these "egg" SmVALs are not involved in direct host-parasite interactions in the mammalian host and are rather proteins employed during intermediate host invasion. Our study revisits and updates the SmVAL gene family, highlighting the limitations of in silico protein function predictions while emphasizing the need for up-to-date datasets and tools together with experimental validation in host-parasite interactions. By uncovering the diversity, expression patterns, and interaction dynamics of SmVALs, we open new avenues for understanding host manipulation and reevaluating orthologous proteins in other helminths.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/GA23-06638S" target="_blank" >GA23-06638S: Sekretované proteiny vajec Schistosoma mansoni: komparativní přístup k identifikaci bioaktivních molekul lidského parazita.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC GENOMICS

ISSN

1471-2164

e-ISSN

1471-2164

Svazek periodika

26

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

16

Strana od-do

—

Kód UT WoS článku

001466847000009

EID výsledku v databázi Scopus

2-s2.0-85218700238