An Ixodes scapularis Protein Disulfide Isomerase Contributes to Borrelia burgdorferi Colonization of the Vector.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F20%3A85197" target="_blank" >RIV/60460709:41340/20:85197 - isvavai.cz</a>

Výsledek na webu

<a href="https://iai.asm.org/content/88/12/e00426-20" target="_blank" >https://iai.asm.org/content/88/12/e00426-20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/IAI.00426-20" target="_blank" >10.1128/IAI.00426-20</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An Ixodes scapularis Protein Disulfide Isomerase Contributes to Borrelia burgdorferi Colonization of the Vector.

Popis výsledku v původním jazyce

Borrelia burgdorferi causes Lyme disease, the most common tick transmitted illness in North America. When Ixodes scapularis feed on an infected vertebrate host, spirochetes enter the tick gut along with the bloodmeal and colonize the vector. Here, we show that a secreted tick protein, I. scapularis protein disulfide isomerase A3 (IsPDIA3), enhances B. burgdorferi colonization of the tick gut. I. scapularis ticks in which ispdiA3 has been knocked down using RNA interference have decreased spirochete colonization of the tick gut after engorging on B. burgdorferi-infected mice. Moreover, administration of IsPDIA3 antiserum to B. burgdorferi infected mice reduced the ability of spirochetes to colonize the tick when feeding on these animals. We show that IsPDIA3 modulates inflammatory responses at the tick bite site, potentially facilitating spirochete survival at the vector host interface as it exits the vertebrate host to enter the tick gut. These data provide functional insights into the complex intera

Název v anglickém jazyce

An Ixodes scapularis Protein Disulfide Isomerase Contributes to Borrelia burgdorferi Colonization of the Vector.

Popis výsledku anglicky

Borrelia burgdorferi causes Lyme disease, the most common tick transmitted illness in North America. When Ixodes scapularis feed on an infected vertebrate host, spirochetes enter the tick gut along with the bloodmeal and colonize the vector. Here, we show that a secreted tick protein, I. scapularis protein disulfide isomerase A3 (IsPDIA3), enhances B. burgdorferi colonization of the tick gut. I. scapularis ticks in which ispdiA3 has been knocked down using RNA interference have decreased spirochete colonization of the tick gut after engorging on B. burgdorferi-infected mice. Moreover, administration of IsPDIA3 antiserum to B. burgdorferi infected mice reduced the ability of spirochetes to colonize the tick when feeding on these animals. We show that IsPDIA3 modulates inflammatory responses at the tick bite site, potentially facilitating spirochete survival at the vector host interface as it exits the vertebrate host to enter the tick gut. These data provide functional insights into the complex intera

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INFECTION AND IMMUNITY

ISSN

0019-9567

e-ISSN

1098-5522

Svazek periodika

88

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

17

Strana od-do

1-17

Kód UT WoS článku

000591527300017

EID výsledku v databázi Scopus

2-s2.0-85096347815