Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede<i> (Brassica</i><i> napus</i> ssp.<i> napobrassica</i> (L.) Hanelt)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F24%3A100111" target="_blank" >RIV/60460709:41340/24:100111 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.17221/84/2023-CJGPB" target="_blank" >https://doi.org/10.17221/84/2023-CJGPB</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/84/2023-CJGPB" target="_blank" >10.17221/84/2023-CJGPB</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede<i> (Brassica</i><i> napus</i> ssp.<i> napobrassica</i> (L.) Hanelt)

Popis výsledku v původním jazyce

Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 mu mol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0-5%), mixoploids especially n+2n were high in all treatments, including the control (29.4-71.1%). Their presence can be explained as a consequence of spontaneous diploidisation and/or endoreduplication during embryo genesis or embryo germination.

Název v anglickém jazyce

Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede<i> (Brassica</i><i> napus</i> ssp.<i> napobrassica</i> (L.) Hanelt)

Popis výsledku anglicky

Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 mu mol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0-5%), mixoploids especially n+2n were high in all treatments, including the control (29.4-71.1%). Their presence can be explained as a consequence of spontaneous diploidisation and/or endoreduplication during embryo genesis or embryo germination.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Genetics and Plant Breeding

ISSN

1212-1975

e-ISSN

1212-1975

Svazek periodika

60

Číslo periodika v rámci svazku

2024

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

79-85

Kód UT WoS článku

001136370800001

EID výsledku v databázi Scopus

2-s2.0-85189289710