DArTseq-generated SNPs revealed low genetic diversity and genetic erosion along life stages in fragmented populations of Afrocarpus gracilior (Pilg.) C. N.Page in southern Ethiopia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F24%3A100910" target="_blank" >RIV/60460709:41340/24:100910 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.foreco.2024.122256" target="_blank" >https://doi.org/10.1016/j.foreco.2024.122256</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foreco.2024.122256" target="_blank" >10.1016/j.foreco.2024.122256</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DArTseq-generated SNPs revealed low genetic diversity and genetic erosion along life stages in fragmented populations of Afrocarpus gracilior (Pilg.) C. N.Page in southern Ethiopia

Popis výsledku v původním jazyce

Forest fragmentation can have severe genetic impacts that threaten the long-term viability of tree species, though susceptibility varies by species. This study investigates the genetic effects of fragmentation on Afrocarpus gracilior populations in traditional agroforestry systems and relict forest patches. We used single nucleotide polymorphism (SNP) markers generated by the DArTseq platform to assess genetic diversity and population structure in both adult and progeny cohorts. Our findings indicate overall low genetic diversity (H-E < 0.1) across all A. gracilior populations studied, with progeny cohorts showing even lower diversity than adults. Progeny from isolated or few mother trees exhibited the lowest genetic diversity, suggesting heightened genetic drift and inbreeding. Genetic differentiation between populations ranged from little (F-ST < 0.05) to moderate (0.05 < F-ST < 0.15), with progeny cohorts from smaller populations showing relatively higher differentiation and significant index of association scores. A Mantel test found no significant correlation between genetic and geographic distances. Analysis of molecular variance indicated that most genetic variation occurred within populations (57-61 %), rather than between populations (1.07-4.93 %) or individuals (about 38 %). Clustering analysis using the discriminant analysis of principal components (DAPC) method, classified the genotypes into five groups, whereas phylogenetic analysis identified three major clusters with further subgrouping. Overall, the study highlights low genetic diversity in A. gracilior populations, with significant inbreeding and genetic drift in progeny from small, isolated populations. These results are crucial for informing conservation, restoration, and genetic rescue efforts for the species.

Název v anglickém jazyce

DArTseq-generated SNPs revealed low genetic diversity and genetic erosion along life stages in fragmented populations of Afrocarpus gracilior (Pilg.) C. N.Page in southern Ethiopia

Popis výsledku anglicky

Forest fragmentation can have severe genetic impacts that threaten the long-term viability of tree species, though susceptibility varies by species. This study investigates the genetic effects of fragmentation on Afrocarpus gracilior populations in traditional agroforestry systems and relict forest patches. We used single nucleotide polymorphism (SNP) markers generated by the DArTseq platform to assess genetic diversity and population structure in both adult and progeny cohorts. Our findings indicate overall low genetic diversity (H-E < 0.1) across all A. gracilior populations studied, with progeny cohorts showing even lower diversity than adults. Progeny from isolated or few mother trees exhibited the lowest genetic diversity, suggesting heightened genetic drift and inbreeding. Genetic differentiation between populations ranged from little (F-ST < 0.05) to moderate (0.05 < F-ST < 0.15), with progeny cohorts from smaller populations showing relatively higher differentiation and significant index of association scores. A Mantel test found no significant correlation between genetic and geographic distances. Analysis of molecular variance indicated that most genetic variation occurred within populations (57-61 %), rather than between populations (1.07-4.93 %) or individuals (about 38 %). Clustering analysis using the discriminant analysis of principal components (DAPC) method, classified the genotypes into five groups, whereas phylogenetic analysis identified three major clusters with further subgrouping. Overall, the study highlights low genetic diversity in A. gracilior populations, with significant inbreeding and genetic drift in progeny from small, isolated populations. These results are crucial for informing conservation, restoration, and genetic rescue efforts for the species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Forest Ecology and Management

ISSN

0378-1127

e-ISSN

0378-1127

Svazek periodika

572

Číslo periodika v rámci svazku

NOV 15 2024

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

—

Kód UT WoS článku

001312769900001

EID výsledku v databázi Scopus

2-s2.0-85203022285