In vitro propagation of Tilia platyphyllos by axillary shoot proliferation and somatic embryogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41410%2F03%3A00006026" target="_blank" >RIV/60460709:41410/03:00006026 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro propagation of Tilia platyphyllos by axillary shoot proliferation and somatic embryogenesis

Popis výsledku v původním jazyce

In vitro propagation of Tilia platyphyllos Scop. has been achieved by axillary shoot proliferation and somatic embryogenesis. The influence of tree age, explant source, genotype, and phytohormones on micropropagation of juvenile and mature trees of Tiliaplatyphyllos has been investigated. Nodal segments and shoot tips were used as initial explants for axillary shoot proliferation. Low concentration of cytokinin (BA, BPA, TDZ) plus auxin (IBA) stimulated fast shoot multiplication. Microshoots excised f rom proliferating cultures were rooted on low salt medium and produced trees were planted in the field. Embryogenic tissues were initiated from zygotic embryos cultured on MS medium suplemented with 2,4-D. After transfer of embryogenic tissues with developing embryoids on media lacking 2,4-D and supplemented with low concetration of IBA, the development of somatic embryos was enhanced. Secondary somatic embryogenesis led to the formation of new adventive somatic embryos. Trees produced fr

Název v anglickém jazyce

In vitro propagation of Tilia platyphyllos by axillary shoot proliferation and somatic embryogenesis

Popis výsledku anglicky

In vitro propagation of Tilia platyphyllos Scop. has been achieved by axillary shoot proliferation and somatic embryogenesis. The influence of tree age, explant source, genotype, and phytohormones on micropropagation of juvenile and mature trees of Tiliaplatyphyllos has been investigated. Nodal segments and shoot tips were used as initial explants for axillary shoot proliferation. Low concentration of cytokinin (BA, BPA, TDZ) plus auxin (IBA) stimulated fast shoot multiplication. Microshoots excised f rom proliferating cultures were rooted on low salt medium and produced trees were planted in the field. Embryogenic tissues were initiated from zygotic embryos cultured on MS medium suplemented with 2,4-D. After transfer of embryogenic tissues with developing embryoids on media lacking 2,4-D and supplemented with low concetration of IBA, the development of somatic embryos was enhanced. Secondary somatic embryogenesis led to the formation of new adventive somatic embryos. Trees produced fr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GK - Lesnictví

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Forest Science

ISSN

1212-4834

e-ISSN

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Svazek periodika

49

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

537-543

Kód UT WoS článku

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EID výsledku v databázi Scopus

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