Inhibice syntézy prostaglandínů prostřednictvím thymochinonu: cyklooxygenasa-1 a -2 testy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41610%2F04%3A9838" target="_blank" >RIV/60460709:41610/04:9838 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The inhibition of prostaglandin biosynthesis by thymoquinone: cyclooxygenase-1 and -2 in vitro assays

Popis výsledku v původním jazyce

The seeds of Nigella sativa L. (Ranunculaceae) known also as black cummin are widely used in traditional medicine in the Middle East and South Asia for the treatment of various respiratory and gastrointestinal diseases, pain in joints and eczema. Recentstudies have demonstrated its anticancer, anti-inflammatory, antihistaminic, antibacterial and analgesic activities. Many of these activities are attributed to quinone constituents, of which thymoquinone is more abundant compound. We report here resultsof an in vitro inhibition of cyclooxygenase-1 (COX-1) and ?2 (COX-2) activity for by thymoquinone, the main quinone compound of N. sativa seeds. The radiochemical assay was based on the inhibition of prostaglandin E2 and D2 biosynthesis from [14C] radioactive arachidonic acid catalyzed by COX-1 and ?2 respectively. The identification and quantification of the COX reaction metabolites were analyzed by HPLC performed on C18 reversed phase column with an on-line radioactivity flow detector.

Název v anglickém jazyce

The inhibition of prostaglandin biosynthesis by thymoquinone: cyclooxygenase-1 and -2 in vitro assays

Popis výsledku anglicky

The seeds of Nigella sativa L. (Ranunculaceae) known also as black cummin are widely used in traditional medicine in the Middle East and South Asia for the treatment of various respiratory and gastrointestinal diseases, pain in joints and eczema. Recentstudies have demonstrated its anticancer, anti-inflammatory, antihistaminic, antibacterial and analgesic activities. Many of these activities are attributed to quinone constituents, of which thymoquinone is more abundant compound. We report here resultsof an in vitro inhibition of cyclooxygenase-1 (COX-1) and ?2 (COX-2) activity for by thymoquinone, the main quinone compound of N. sativa seeds. The radiochemical assay was based on the inhibition of prostaglandin E2 and D2 biosynthesis from [14C] radioactive arachidonic acid catalyzed by COX-1 and ?2 respectively. The identification and quantification of the COX reaction metabolites were analyzed by HPLC performed on C18 reversed phase column with an on-line radioactivity flow detector.

Druh

D - Stať ve sborníku

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F02%2F0257" target="_blank" >GA525/02/0257: Stanovení antibakteriální a protizánětlivé aktivity vybraných druhů rodu Nigella L.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník abstraktů: International Congress on Natural Product Research

ISBN

nemá

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

1-1

Název nakladatele

International Congress on Natural Product Research

Místo vydání

Phoenix, Arizona, USA

Místo konání akce

Phoenix, Arizona, USA

Datum konání akce

31. 7. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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