Immunochemický screening izoflavonů v čeledi Rutaceae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41610%2F04%3A9841" target="_blank" >RIV/60460709:41610/04:9841 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immunochemical screening of isoflavones in the Rutaceae family

Popis výsledku v původním jazyce

Isoflavones (3-phenyl chromones) are phytoalexins abundant in the Fabaceae, however their biosynthesis was described in over 20 other families, e.g. Rosaceae, Iridaceae, Asteraceae, Poaceae. Molecular biology data indicate occurrence of genes for isoflavonoid metabolic pathway even in higher number of families (1), although it is difficult to obtain the evidence of their biochemical activity. Recently, we have found isoflavonoids in the Rutaceae family (1). In our recent research, we combine immunochemical approaches and HPLC-MS. EXPERIMENTAL: Five immunoassays for early products of the isoflavonoid pathway (i.e. daidzein, formononetin, genistein and biochanin A and their glycosides) were adapted for phytochemical studies. Tested plant material (leavesfrom apical branches) was frozen immediately after collecting and kept in ?20 °C until lyophilized. A limited number of samples were divided to two aliquots of which one was dried in laboratory temperature in shadow. Dry samples were pul

Název v anglickém jazyce

Immunochemical screening of isoflavones in the Rutaceae family

Popis výsledku anglicky

Isoflavones (3-phenyl chromones) are phytoalexins abundant in the Fabaceae, however their biosynthesis was described in over 20 other families, e.g. Rosaceae, Iridaceae, Asteraceae, Poaceae. Molecular biology data indicate occurrence of genes for isoflavonoid metabolic pathway even in higher number of families (1), although it is difficult to obtain the evidence of their biochemical activity. Recently, we have found isoflavonoids in the Rutaceae family (1). In our recent research, we combine immunochemical approaches and HPLC-MS. EXPERIMENTAL: Five immunoassays for early products of the isoflavonoid pathway (i.e. daidzein, formononetin, genistein and biochanin A and their glycosides) were adapted for phytochemical studies. Tested plant material (leavesfrom apical branches) was frozen immediately after collecting and kept in ?20 °C until lyophilized. A limited number of samples were divided to two aliquots of which one was dried in laboratory temperature in shadow. Dry samples were pul

Druh

D - Stať ve sborníku

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F03%2F0352" target="_blank" >GA525/03/0352: Isoflavonoidy v čeledích Myrtaceae a Rutaceae</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

19th Meeting of Czech and Slovak Societies for Biochemistry and Molecular Biology

ISBN

80-244-0882-1

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

215-216

Název nakladatele

Univerzita Palackého

Místo vydání

Olomouc, Czech Republic

Místo konání akce

Olomouc, Czech Republic

Datum konání akce

31. 8. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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